In vitro study on the dimensional changes of human dentine after demineralization

R. M. Carvalho, M. Yoshiyama, E. L. Pashley, D. H. Pashley

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99 Citations (Scopus)


Dentine rods measuring approximately 0.7 × 0.7 × 5.0 mm were prepared from dentine of extracted human third molars stored in saline containing 0.5% sodium azide at 4°C until used. Forty specimens were demineralized in 10% citric acid plus 3% ferric chloride (w/w) solution for 8h, then assigned to four groups (A, B, C and D) of 10 specimens each. Groups A and B were used to investigate volumetric changes after air-drying and further immersion in either water, an aqueous solution of 50% 2-hydroxymethylmethacrylate (HEMA) or 100% HEMA, followed by air-drying. Groups C and D were used to investigate the ability of 100% HEMA or 100% ethylene glycol to prevent shrinkage of demineralized dentine during exposure to air. Demineralization caused a small, non-significant (1.9%) reduction in dentine volume. Air-drying further reduced the volume by 65.6%. When demineralized, shrunken specimens were immersed in water for 24 h, they recovered their original demineralized volume. Immersion in 100% HEMA did not re-expand demineralized shrunken dentine. Specimens immersed in 50% HEMA yielded a 50% volume shrinkage when exposed to air for 24 h. Both 100% HEMA and 100% ethylene glycol were effective in preventing shrinkage of demineralized dentine. The technique used provided useful information about maximal dimensional changes that may occur at a microscopic level during adhesive dental restorative procedures.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalArchives of Oral Biology
Issue number4
Publication statusPublished - 1996
Externally publishedYes


  • Demineralized dentine
  • Dentine
  • Shrinkage

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry
  • Cell Biology


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