@inbook{acd1c34eb2c84b9b9cd9c377ffa03799,
title = "In vitro transfection with and expression of CCN family of genes",
abstract = "The ability to engineer cells to express a protein of interest in an inducible manner and stably for a long period is a valuable tool in molecular biology and also one that holds promise for regenerative medicine in the future. CCN proteins have been suggested to be involved in tissue regeneration. In this chapter, we describe an in vitro method for stable and inducible expression of CCN protein in a chondroprogenitor cell line and in chondrocytes in primary culture that does not involve the use of any viral vector.",
keywords = "CCN, Expression, PiggyBac, Stable, Transposon",
author = "Danilo Janune and Masaharu Takigawa",
note = "Funding Information: The authors would like to thank Yoshiko Miyake for valuable secretarial assistance. D.J. was a postdoctoral fellow supported by the Japanese Society for Promotion of Science (JSPS) when he established this method. The experiments pertaining to the establishment of this method were supported by a grant-in-aid attached to the JSPS Postdoctoral Fellowship for Overseas Researchers (number 25/03412) to M.T. and D.J. and by grants from the program Grantsin-aid for Scientific Research (B) [#JP24390415 and #JP15H05014] to M.T. from the Japan Society for the Promotion of Science. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_11",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "107--113",
booktitle = "Methods in Molecular Biology",
}