In vitro transfection with and expression of CCN family of genes

Danilo Janune, Masaharu Takigawa

Research output: Chapter in Book/Report/Conference proceedingChapter


The ability to engineer cells to express a protein of interest in an inducible manner and stably for a long period is a valuable tool in molecular biology and also one that holds promise for regenerative medicine in the future. CCN proteins have been suggested to be involved in tissue regeneration. In this chapter, we describe an in vitro method for stable and inducible expression of CCN protein in a chondroprogenitor cell line and in chondrocytes in primary culture that does not involve the use of any viral vector.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages7
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • CCN
  • Expression
  • PiggyBac
  • Stable
  • Transposon

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'In vitro transfection with and expression of CCN family of genes'. Together they form a unique fingerprint.

Cite this