In vivo evaluation of cartilage regenerative effects of CCN2 protein

Takashi Nishida, Satoshi Kubota, Masaharu Takigawatakigawa

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique growth factor that promotes the proliferation and differentiation, but not the hypertrophy of articular chondrocytes in vitro. Based on these fi ndings, we previously evaluated the cartilage-regenerative effects of recombinant CCN2 protein (rCCN2) by using both mono-iodoacetate (MIA) injection into the rat joint cavity and formation of full-thickness defects of rat articular cartilage in vivo, and our results suggested the utility of CCN2 in the regeneration of articular cartilage. This chapter entails helpful tips to apply these two animal models for the evaluation of cartilage-regenerative effects of CCN2 or its derivatives.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages273-282
Number of pages10
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

Keywords

  • Articular cartilage
  • Collagen sponge
  • Full-thickness defects
  • Gelatin hydrogels
  • Micro drill
  • Mono-iodoacetic acid (MIA)
  • Recombinant CCN2 protein (rCCN2)
  • Regeneration and repair

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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