@inbook{10130d41319841209bbd2109f60a1e83,
title = "In vivo-like cell-cycle phase distribution of cancer cells in Gelfoam{\textregistered} histoculture observed in real time by FUCCI imaging",
abstract = "FUCCI color codes cells as they express different color fluorescent proteins as they go through phases of the cell cycle. The cell cycle phase distribution of cancer cells in Gelfoam{\textregistered} histoculture was similar to in vivo tumors, whereby only the surface cells proliferate and interior cells are quiescent in G0/G1. In contrast, in 2D cancer-cell culture, most of the cells are always cycling. The cancer cells responded similarly to toxic chemotherapy in Gelfoam{\textregistered} culture as in vivo; as such, therapy is cell cycle dependent. In 2D culture, cancer cells were much more chemosensitive. These results indicate why the drug response pattern of tumors in Gelfoam{\textregistered} histoculture reflects what is observed in vivo.",
keywords = "3D culture, Cell cycle, Chemotherapy, Color-coding, FUCCI (fluorescence ubiquitination cell cycle indicator), Gelfoam histoculture, Imaging",
author = "Hoffman, {Robert M.} and Shuya Yano",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-7745-1_12",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "109--123",
booktitle = "Methods in Molecular Biology",
}
