TY - JOUR
T1 - Inactivation of the mitogenic property of pokeweed mitogen by erythrocytes
AU - Ohno, Ryuzo
AU - Kodera, Yoshihisa
AU - Ezaki, Kohji
AU - Tanimoto, Mitsune
AU - Iwamoto, Kohji
AU - Yamada, Kazumasa
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture. We express our sincere gratitude to Miss Sayoko Sugiura for her excellent technical assistance.
PY - 1979/12/14
Y1 - 1979/12/14
N2 - In whole blood culture, pokeweed mitogen (PWM) is unable to stimulate human lymphocytes. This is because the mitogenic property of PWM is inactivated by erythrocytes, presumably due to absorption. The inactivation was observed with as few as 5 × 106/ml of human erythrocytes. Therefore, when PWM is used to study the functions of human lymphocytes, especially of suppressor T lymphocytes, erythrocytes should be removed from lymphocyte preparations for accurate analysis.
AB - In whole blood culture, pokeweed mitogen (PWM) is unable to stimulate human lymphocytes. This is because the mitogenic property of PWM is inactivated by erythrocytes, presumably due to absorption. The inactivation was observed with as few as 5 × 106/ml of human erythrocytes. Therefore, when PWM is used to study the functions of human lymphocytes, especially of suppressor T lymphocytes, erythrocytes should be removed from lymphocyte preparations for accurate analysis.
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U2 - 10.1016/0022-1759(79)90286-2
DO - 10.1016/0022-1759(79)90286-2
M3 - Article
C2 - 512376
AN - SCOPUS:0018639615
SN - 0022-1759
VL - 31
SP - 65
EP - 69
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -