TY - JOUR
T1 - Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production
AU - Ookawa, Taiichiro
AU - Inoue, Kazuya
AU - Matsuoka, Makoto
AU - Ebitani, Takeshi
AU - Takarada, Takeshi
AU - Yamamoto, Toshio
AU - Ueda, Tadamasa
AU - Yokoyama, Tadashi
AU - Sugiyama, Chisato
AU - Nakaba, Satoshi
AU - Funada, Ryo
AU - Kato, Hiroshi
AU - Kanekatsu, Motoki
AU - Toyota, Koki
AU - Motobayashi, Takashi
AU - Vazirzanjani, Mehran
AU - Tojo, Seishu
AU - Hirasawa, Tadashi
N1 - Funding Information:
This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics-based for Agricultural Improvement, RBS2003).
PY - 2014
Y1 - 2014
N2 - Lignin modification has been a breeding target for the improvements of forage digestibility and energy yields in forage and bioenergy crops, but decreased lignin levels are often accompanied by reduced lodging resistance. The rice mutant gold hull and internode2 (gh2) has been identified to be lignin deficient. GH2 has been mapped to the short arm of chromosome 2 and encodes cinnamyl-alcohol dehydrogenase (CAD). Wedeveloped a long-culm variety, 'Leaf Star', with superior lodging resistance and agh phenotype similar to one of its parents, 'Chugoku 117'. Thegh loci in Leaf Star and Chugoku 117 were localized to the same region of chromosome 2 as the gh2 mutant. Leaf Star had culms with low lignin concentrations due to a natural mutation in OsCAD2 that was not present in Chugoku 117. However, this variety had high culm strength due to its strong, thick culms. Additionally, this variety had a thick layer of cortical fiber tissue with well-developed secondary cell walls. Our results suggest that rice can be improved for forage and bioenergy production by combining superior lodging resistance, which can be obtained by introducing thick and stiff culm traits, with low lignin concentrations, which can be obtained using the gh2 variety.
AB - Lignin modification has been a breeding target for the improvements of forage digestibility and energy yields in forage and bioenergy crops, but decreased lignin levels are often accompanied by reduced lodging resistance. The rice mutant gold hull and internode2 (gh2) has been identified to be lignin deficient. GH2 has been mapped to the short arm of chromosome 2 and encodes cinnamyl-alcohol dehydrogenase (CAD). Wedeveloped a long-culm variety, 'Leaf Star', with superior lodging resistance and agh phenotype similar to one of its parents, 'Chugoku 117'. Thegh loci in Leaf Star and Chugoku 117 were localized to the same region of chromosome 2 as the gh2 mutant. Leaf Star had culms with low lignin concentrations due to a natural mutation in OsCAD2 that was not present in Chugoku 117. However, this variety had high culm strength due to its strong, thick culms. Additionally, this variety had a thick layer of cortical fiber tissue with well-developed secondary cell walls. Our results suggest that rice can be improved for forage and bioenergy production by combining superior lodging resistance, which can be obtained by introducing thick and stiff culm traits, with low lignin concentrations, which can be obtained using the gh2 variety.
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U2 - 10.1038/srep06567
DO - 10.1038/srep06567
M3 - Article
C2 - 25298209
AN - SCOPUS:84923357339
SN - 2045-2322
VL - 4
JO - Scientific reports
JF - Scientific reports
M1 - 6567
ER -