Induction and characterization of cutaneous lymphocyte antigen on natural killer cells

Junjiro Tsuchiyama, Tadashi Yoshino, Ken Toba, Naoki Harada, Ritsuo Nishiuchi, Tadaatsu Akagi, Tatsuo Furukawa, Masuiro Takahashi, Ichiro Fuse, Yoshifusa Aizawa, Mine Harada

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Cutaneous lymphocyte antigen (CLA) has been reported to be expressed mainly by memory/effector T lymphocytes infiltrating inflammatory skin lesions and cutaneous T-cell lymphoma. It has been suggested that CLA is a specific homing receptor, facilitating the T-cell migration into skin lesions, and also an indicator of the skin-homing T-cell subset. In the present study, we investigated the expression of CLA in natural killer (NK) cells defined phenotypically as surface CD3- and CD56+ cells in peripheral blood. CLA was definitely expressed on CD3-CD56+ cells at a level comparable to CD3+ cells in peripheral blood of normal Japanese volunteers. After in vitro stimulation of peripheral blood mononuclear cells with interleukin 2 (IL-2) and IL-12, there was a significant increase in the number and percentage of CLA+ NK cells but not CLA+ T cells (P < 0.01). To analyse the characteristics of CLA expressed by NK cells, we investigated a CLA+ NK-leukaemia cell line, NK-YS, established from a patient with NK leukaemia/lymphoma with skin infiltration. In the in vitro study, the CLA-expressing NK-leukaemic cell line bound to E-selectin-transfected cells and was inhibited by HECA 452 antibody or neuraminidase treatment of leukaemic cells. These findings suggest that CLA expressed by NK cells is a homing receptor for the E-selectin molecule and may explain skin infiltration by NK cells and NK lymphoma cells analogous to T cells. An NK-cell subset expressing CLA must play an important role in host defence against microorganisms and neoplasms in skin lesions.

Original languageEnglish
Pages (from-to)654-662
Number of pages9
JournalBritish Journal of Haematology
Issue number2
Publication statusPublished - 2002


  • Homing receptor
  • NK cells
  • Skin involvement

ASJC Scopus subject areas

  • Hematology


Dive into the research topics of 'Induction and characterization of cutaneous lymphocyte antigen on natural killer cells'. Together they form a unique fingerprint.

Cite this