TY - JOUR
T1 - Induction of cemip in chondrocytes by inflammatory cytokines
T2 - Underlying mechanisms and potential involvement in osteoarthritis
AU - Ohtsuki, Takashi
AU - Hatipoglu, Omer F.
AU - Asano, Keiichi
AU - Inagaki, Junko
AU - Nishida, Keiichiro
AU - Hirohata, Satoshi
N1 - Funding Information:
Funding: This work was supported in part by a Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (nos.17K19727 and 17H04313 to S.H., 19K09627 to T.O., and 19K11791 to J.I.).
Publisher Copyright:
© 2020 by the authors.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - In patients with osteoarthritis (OA), there is a decrease in both the concentration and molecular size of hyaluronan (HA) in the synovial fluid and cartilage. Cell migration-inducing hyaluronidase 1 (CEMIP), also known as hyaluronan (HA)-binding protein involved in HA depolymerization (HYBID), was recently reported as an HA depolymerization-related molecule expressed in the cartilage of patients with OA. However, the underlying mechanism of CEMIP regulation is not well understood. We found that CEMIP expression was transiently increased by interleukine-1β (IL-1β) stimulation in chondrocytic cells. We also observed that ERK activation and NF-κB nuclear translocation were involved in the induction of CEMIP by IL-1β. In addition, both administration of HA and mechanical strain attenuated the CEMIP induction in IL-1β-stimulated chondrocytes. In conclusion, we clarified the regulatory mechanism of CEMIP in chondrocytes by inflammatory cytokines and suggested the potential involvement in osteoarthritis development.
AB - In patients with osteoarthritis (OA), there is a decrease in both the concentration and molecular size of hyaluronan (HA) in the synovial fluid and cartilage. Cell migration-inducing hyaluronidase 1 (CEMIP), also known as hyaluronan (HA)-binding protein involved in HA depolymerization (HYBID), was recently reported as an HA depolymerization-related molecule expressed in the cartilage of patients with OA. However, the underlying mechanism of CEMIP regulation is not well understood. We found that CEMIP expression was transiently increased by interleukine-1β (IL-1β) stimulation in chondrocytic cells. We also observed that ERK activation and NF-κB nuclear translocation were involved in the induction of CEMIP by IL-1β. In addition, both administration of HA and mechanical strain attenuated the CEMIP induction in IL-1β-stimulated chondrocytes. In conclusion, we clarified the regulatory mechanism of CEMIP in chondrocytes by inflammatory cytokines and suggested the potential involvement in osteoarthritis development.
KW - Cell migration-inducing hyaluronidase 1 (CEMIP)
KW - Chondrocyte
KW - Hyaluronan
KW - Mechanical strain
KW - Nuclear factor kappa B (NF-κB)
KW - Osteoarthritis
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U2 - 10.3390/ijms21093140
DO - 10.3390/ijms21093140
M3 - Article
C2 - 32365591
AN - SCOPUS:85084277121
SN - 1661-6596
VL - 21
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 9
M1 - 3140
ER -