TY - JOUR
T1 - Induction of glial cell line-derived neurotrophic factor receptor proteins in cerebral cortex and striatum after permanent middle cerebral artery occlusion in rats
AU - Kitagawa, Hisashi
AU - Sasaki, Chihoko
AU - Zhang, Wen Ri
AU - Sakai, Kenichi
AU - Shiro, Yoshihiko
AU - Warita, Hitoshi
AU - Mitsumoto, Yasuhide
AU - Mori, Toyoki
AU - Abe, Koji
N1 - Funding Information:
This work was partly supported by Grants-in-Aid for Scientific Research on 09470151 and 10176204 from the Ministry of Education, Japan.
PY - 1999/7/10
Y1 - 1999/7/10
N2 - In an attempt to elucidate whether glial cell line-derived neurotrophic factor (GDNF) receptors are induced after ischemic brain injury, possible expression of immunoreactive GDNF receptor-α1 (GFRα-1) and c-ret (RET) was examined at 3, 8, or 24 h after permanent middle cerebral artery occlusion (MCAO) in rats. Immunohistochemical study showed that both GFRα-1 and RET staining cells which were not detected in sham control brain, were present in the ipsilateral cortex and caudate at 3 to 8 h after permanent MCAO, and then decreased but remained to some extent at 24 h. Positive cells for both GDNF receptors were predominantly in cortical neurons of ischemic penumbral area. Western blot analysis confirmed the induction of those receptors after permanent MCAO. This rapid induction of GFRα-1 and RET, which correlates with the similar induction of GDNF under these conditions, may play a role in the early response to ischemic brain injury.
AB - In an attempt to elucidate whether glial cell line-derived neurotrophic factor (GDNF) receptors are induced after ischemic brain injury, possible expression of immunoreactive GDNF receptor-α1 (GFRα-1) and c-ret (RET) was examined at 3, 8, or 24 h after permanent middle cerebral artery occlusion (MCAO) in rats. Immunohistochemical study showed that both GFRα-1 and RET staining cells which were not detected in sham control brain, were present in the ipsilateral cortex and caudate at 3 to 8 h after permanent MCAO, and then decreased but remained to some extent at 24 h. Positive cells for both GDNF receptors were predominantly in cortical neurons of ischemic penumbral area. Western blot analysis confirmed the induction of those receptors after permanent MCAO. This rapid induction of GFRα-1 and RET, which correlates with the similar induction of GDNF under these conditions, may play a role in the early response to ischemic brain injury.
KW - Focal ischemia
KW - GFRα-1
KW - Immunoreactivity
KW - Middle cerebral artery occlusion
KW - Ret
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U2 - 10.1016/S0006-8993(99)01563-2
DO - 10.1016/S0006-8993(99)01563-2
M3 - Article
C2 - 10407114
AN - SCOPUS:0033542742
SN - 0006-8993
VL - 834
SP - 190
EP - 195
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -