Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1

Yuji Morita; Takeshi Murata; Takehiko Mima; Sumiko Shiota; Teruo Kuroda; Tohru Mizushima; Naomasa Gotoh; Takeshi Nishino; Tomofusa Tsuchiya

doi:10.1093/jac/dkg173

Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1

Yuji Morita, Takeshi Murata, Takehiko Mima, Sumiko Shiota, Teruo Kuroda, Tohru Mizushima, Naomasa Gotoh, Takeshi Nishino, Tomofusa Tsuchiya

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

Induction of the MexCD-OprJ multidrug efflux pump was investigated in wild-type Pseudomonas aeruginosa PAO1. MexCD-OprJ was induced by clinically important disinfectants such as benzalkonium chloride and chlorhexidine gluconate, and by some cytotoxic agents such as tetraphenylphosphonium chloride, ethidium bromide and rhodamine 6G. MexCD-OprJ was not induced by norfloxacin, tetracycline, chloramphenicol, streptomycin, erythromycin or carbenicillin, although they are substrates for the pump. Cells of PAO1 showed increased resistance to norfloxacin when grown in the presence of the inducers of the mexCD-oprJ operon mentioned above. These results indicate that MexCD-OprJ plays an important role in intrinsic multidrug resistance in wild-type P. aeruginosa in hospitals where disinfectants are used frequently.

Original language	English
Pages (from-to)	991-994
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	51
Issue number	4
DOIs	https://doi.org/10.1093/jac/dkg173
Publication status	Published - Apr 1 2003

Keywords

Inducers
MexCD-OprJ
Multidrug efflux pump
P. aeruginosa

ASJC Scopus subject areas

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jac/dkg173

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title = "Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1",

abstract = "Induction of the MexCD-OprJ multidrug efflux pump was investigated in wild-type Pseudomonas aeruginosa PAO1. MexCD-OprJ was induced by clinically important disinfectants such as benzalkonium chloride and chlorhexidine gluconate, and by some cytotoxic agents such as tetraphenylphosphonium chloride, ethidium bromide and rhodamine 6G. MexCD-OprJ was not induced by norfloxacin, tetracycline, chloramphenicol, streptomycin, erythromycin or carbenicillin, although they are substrates for the pump. Cells of PAO1 showed increased resistance to norfloxacin when grown in the presence of the inducers of the mexCD-oprJ operon mentioned above. These results indicate that MexCD-OprJ plays an important role in intrinsic multidrug resistance in wild-type P. aeruginosa in hospitals where disinfectants are used frequently.",

keywords = "Inducers, MexCD-OprJ, Multidrug efflux pump, P. aeruginosa",

author = "Yuji Morita and Takeshi Murata and Takehiko Mima and Sumiko Shiota and Teruo Kuroda and Tohru Mizushima and Naomasa Gotoh and Takeshi Nishino and Tomofusa Tsuchiya",

note = "Funding Information: We thank Dr M. Varela of Eastern New Mexico University for critical reading of the manuscript. This research was supported in part by a grant from the Ministry of Education, Science, Sport and Culture of Japan.",

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doi = "10.1093/jac/dkg173",

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AU - Morita, Yuji

AU - Murata, Takeshi

AU - Mima, Takehiko

AU - Shiota, Sumiko

AU - Kuroda, Teruo

AU - Mizushima, Tohru

AU - Gotoh, Naomasa

AU - Nishino, Takeshi

AU - Tsuchiya, Tomofusa

N1 - Funding Information: We thank Dr M. Varela of Eastern New Mexico University for critical reading of the manuscript. This research was supported in part by a grant from the Ministry of Education, Science, Sport and Culture of Japan.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Induction of the MexCD-OprJ multidrug efflux pump was investigated in wild-type Pseudomonas aeruginosa PAO1. MexCD-OprJ was induced by clinically important disinfectants such as benzalkonium chloride and chlorhexidine gluconate, and by some cytotoxic agents such as tetraphenylphosphonium chloride, ethidium bromide and rhodamine 6G. MexCD-OprJ was not induced by norfloxacin, tetracycline, chloramphenicol, streptomycin, erythromycin or carbenicillin, although they are substrates for the pump. Cells of PAO1 showed increased resistance to norfloxacin when grown in the presence of the inducers of the mexCD-oprJ operon mentioned above. These results indicate that MexCD-OprJ plays an important role in intrinsic multidrug resistance in wild-type P. aeruginosa in hospitals where disinfectants are used frequently.

AB - Induction of the MexCD-OprJ multidrug efflux pump was investigated in wild-type Pseudomonas aeruginosa PAO1. MexCD-OprJ was induced by clinically important disinfectants such as benzalkonium chloride and chlorhexidine gluconate, and by some cytotoxic agents such as tetraphenylphosphonium chloride, ethidium bromide and rhodamine 6G. MexCD-OprJ was not induced by norfloxacin, tetracycline, chloramphenicol, streptomycin, erythromycin or carbenicillin, although they are substrates for the pump. Cells of PAO1 showed increased resistance to norfloxacin when grown in the presence of the inducers of the mexCD-oprJ operon mentioned above. These results indicate that MexCD-OprJ plays an important role in intrinsic multidrug resistance in wild-type P. aeruginosa in hospitals where disinfectants are used frequently.

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KW - MexCD-OprJ

KW - Multidrug efflux pump

KW - P. aeruginosa

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Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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