Induction of monocyte chemoattractant protein 1 by heticobacter pylori involves NF-κB

N. Mori, A. Ueda, R. Geleziunas, A. Wada, T. Hirayama, T. Yoshimura, N. Yamamoto

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Helicobacter pylori stimulates secretion of monocyle chemoattractant protein 1 (MCP-1) from gastric epithelial cells. Secretion of this chemokine may be instrumental in monocyte infiltration of the gastric epithelium that characterizes H. pylori gastritis. The aim of this study was to identify the mechanism by which H. pylori induces MCP-1 production. Induction of MCP-1 mRNA was assessed by reverse transcription-PCR. We used luciferase reporter assays to monitor activation of the MCP-1 gene promoter and electrophoretic mobility shift assays to explore binding of transcription factors to this promoter. H. pylori infection increased MCP-1 mRNA expression from gastric epithelial cells. Induction of MCP-1 mRNA relies on an intact cag pathogenicity island. We identified two closely spaced NF-κB-binding sites within the MCP-1 distal enhancer as required for H. pylori-induced MCP-1 gene transcription. H. pylori infection led to the specific activation of NF-κB complexes containing p50 and p65. Kinase-deficient mutants of NF-κB-inducing kinase (NIK) and IκB kinases (IKK) caused suppression of MCP-1 distal enhancer-dependent reporter activity following H. pylori infection. H. pylori infection induces the activation of NF-κB via the NIK-IKK signaling complex, leading to MCP-1 gene transcription in gastric epithelial cells.

Original languageEnglish
Pages (from-to)1280-1286
Number of pages7
JournalInfection and Immunity
Issue number3
Publication statusPublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'Induction of monocyte chemoattractant protein 1 by heticobacter pylori involves NF-κB'. Together they form a unique fingerprint.

Cite this