Induction of sex change within two full moons during breeding season and spawning in grouper

Mohammad Ashraful Alam, Ramji Kumar Bhandari, Yasuhisa Kobayashi, Kiyoshi Soyano, Masaru Nakamura

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Artificial sex change was induced within two full moons by an aromatase inhibitor (AI; 1 mg/fish) during the breeding season to establish the quickest method of sex change and natural spawning of the honeycomb grouper (Epinephelus merra). The sex change from female (ovary) to male (testis) occurred during the time between the two full moons (3 weeks) following AI implantation, and the efferent ducts of sex-changed males were filled with sperm. To examine sperm fertility, sex-changed males were mated with natural, normal females and produced fertilized eggs. Most of the hatched larvae grew normally without any morphological deformities. Therefore, the use of this method which is the quickest known sex change method using AI, may contribute to quality sperm production for grouper aquaculture.

Original languageEnglish
Pages (from-to)532-535
Number of pages4
Issue number1-4
Publication statusPublished - May 31 2006


  • Artificial sex change
  • Breeding season
  • Full moon
  • Honeycomb grouper
  • Spawning

ASJC Scopus subject areas

  • Aquatic Science


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