Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus

Miwako Todoroki, Yoichi Ueta, Hiroaki Fujihara, Hiroki Otsubo, Minori Shibata, Hirofumi Hashimoto, Mizuki Kobayashi, Hirotaka Sakamoto, Mitsuhiro Kawata, Govindan Dayanithi, David Murphy, Hisanori Hiro, Ken Takahashi, Shoji Nagata

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 1248 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress.

Original languageEnglish
Pages (from-to)281-292
Number of pages12
Issue number4
Publication statusPublished - Jul 2010
Externally publishedYes


  • Colchicine
  • Green fluorescent protein
  • Hypothalamus
  • Locus coeruleus
  • Noradrenaline
  • Stress
  • Vasopressin

ASJC Scopus subject areas

  • Physiology
  • Neuropsychology and Physiological Psychology
  • Endocrine and Autonomic Systems
  • Psychiatry and Mental health
  • Behavioral Neuroscience


Dive into the research topics of 'Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus'. Together they form a unique fingerprint.

Cite this