TY - JOUR
T1 - Inductions of Cu/Zn superoxide dismutase- and nitric oxide synthase-like immunoreactivities in rabbit spinal cord after transient ischemia
AU - Watanabe, M.
AU - Sakurai, M.
AU - Abe, K.
AU - Aoki, M.
AU - Sadahiro, M.
AU - Tabayashi, K.
AU - Okamoto, K.
AU - Shoji, M.
AU - Itoyama, Y.
N1 - Funding Information:
We thank Dr. Atsushi Sakurai and Mrs. Etsuko Sato for their technical assistance. This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas (Kanazawa, I.) 06272204 and 07264204, Grant-in-Aid for Scientific Research (C) 06807055 from the Ministry of Education, Science and Culture of Japan, grants (Hirai, S., Yanagisawa, N.) from the Ministry of Health and Welfare of Japan, and the grant provided by the Ichiro Kanehara Foundation.
PY - 1996/9/2
Y1 - 1996/9/2
N2 - The distributions and inductions of Cu/Zn superoxide dismutase (SOD), neuronal and endothelial nitric oxide (NO) synthase (nNOS and eNOS), and nitrotyrosine (NT) were immunohistochemically examined in rabbit spinal cords after 5 and 15 min of transient ischemia. The neurons in the anterior horns (AH) were selectively lost 7 days after 15-min ischemia as compared with those of sham-operated controls. In the normal spinal cords, a number of neurons in the AHs were positive for the nNOS, and only slightly positive for the Cu/Zn SOD and the eNOS. Immunoreactivities for the proteins were induced at 8-24 h both after 5- and 15-min ischemia. In contrast, NT-like immunoreactivity was negative both in the normal and postischemic spinal cords. These results suggest that Cu/Zn SOD- and nNOS-, and eNOS-like immunoreactivities are induced, but that, even though an interaction of Cu/Zn SOD with NO could be present, NT was not detected in the motor neurons in the rabbit spinal cords after transient ischemia. Other factors could be required for NT formation found in degenerative motor neuron death in humans.
AB - The distributions and inductions of Cu/Zn superoxide dismutase (SOD), neuronal and endothelial nitric oxide (NO) synthase (nNOS and eNOS), and nitrotyrosine (NT) were immunohistochemically examined in rabbit spinal cords after 5 and 15 min of transient ischemia. The neurons in the anterior horns (AH) were selectively lost 7 days after 15-min ischemia as compared with those of sham-operated controls. In the normal spinal cords, a number of neurons in the AHs were positive for the nNOS, and only slightly positive for the Cu/Zn SOD and the eNOS. Immunoreactivities for the proteins were induced at 8-24 h both after 5- and 15-min ischemia. In contrast, NT-like immunoreactivity was negative both in the normal and postischemic spinal cords. These results suggest that Cu/Zn SOD- and nNOS-, and eNOS-like immunoreactivities are induced, but that, even though an interaction of Cu/Zn SOD with NO could be present, NT was not detected in the motor neurons in the rabbit spinal cords after transient ischemia. Other factors could be required for NT formation found in degenerative motor neuron death in humans.
KW - Cu/Zn superoxide dismutase
KW - motor neuron death
KW - nitric oxide synthase
KW - nitrotyrosine
KW - rabbit
KW - spinal ischemia
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U2 - 10.1016/0006-8993(96)00490-8
DO - 10.1016/0006-8993(96)00490-8
M3 - Article
C2 - 8891270
AN - SCOPUS:0030565537
SN - 0006-8993
VL - 732
SP - 69
EP - 74
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -