Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from thermosynechococcus vulcanus

Keisuke Saito; Jian Ren Shen; Hiroshi Ishikita

doi:10.1016/j.bpj.2012.04.016

Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from thermosynechococcus vulcanus

Keisuke Saito, Jian Ren Shen, Hiroshi Ishikita

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Influence of the axial ligand of P_D1 chlorophyll (D1-His-198) on the E_m of monomer chlorophylls P_D1 and P_D2, and the P_D1^•+/P_D2^•+ charge ratio was investigated by theoretical calculations using the PSII crystal structure of Thermosynechococcus vulcanus analyzed at 1.9-Å resolution. It was found that the E_m(P_D1)/E_m(P_D2) values and P_D1^•+/P_D2^•+ ratio remained unchanged upon D1-H198Q mutation. However, E_m(P _D1) was increased in the D1-H198A mutant, resulting in a more even distribution of the positive charge over P_D1/P_D2. Introduction of a water molecule as an axial ligand resulted in equal E _m values and P_D1^•+/P_D2 ^•+ ratios between the mutant and wild-type, thus confirming the presence of the water ligand in the mutant.

Original language	English
Pages (from-to)	2634-2640
Number of pages	7
Journal	Biophysical Journal
Volume	102
Issue number	11
DOIs	https://doi.org/10.1016/j.bpj.2012.04.016
Publication status	Published - Jun 6 2012

ASJC Scopus subject areas

Biophysics

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10.1016/j.bpj.2012.04.016

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title = "Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from thermosynechococcus vulcanus",

abstract = "Influence of the axial ligand of PD1 chlorophyll (D1-His-198) on the Em of monomer chlorophylls PD1 and PD2, and the PD1•+/PD2•+ charge ratio was investigated by theoretical calculations using the PSII crystal structure of Thermosynechococcus vulcanus analyzed at 1.9-{\AA} resolution. It was found that the Em(PD1)/Em(PD2) values and PD1•+/PD2•+ ratio remained unchanged upon D1-H198Q mutation. However, Em(P D1) was increased in the D1-H198A mutant, resulting in a more even distribution of the positive charge over PD1/PD2. Introduction of a water molecule as an axial ligand resulted in equal E m values and PD1•+/PD2 •+ ratios between the mutant and wild-type, thus confirming the presence of the water ligand in the mutant.",

author = "Keisuke Saito and Shen, {Jian Ren} and Hiroshi Ishikita",

note = "Funding Information: This research was supported by the Japan Science and Technology Precursory Research for Embryonic Science and Technology (JST PRESTO) program (to H.I.), Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (22740276 to K.S.), Special Coordination Fund (to H.I.) for Promoting Science and Technology of MEXT, Takeda Science Foundation (to H.I.), Kyoto University Step-up Grant-in-Aid for young scientists (to H.I.), and Grant for Basic Science Research Projects from The Sumitomo Foundation (to H.I.). ",

year = "2012",

month = jun,

day = "6",

doi = "10.1016/j.bpj.2012.04.016",

language = "English",

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journal = "Biophysical Journal",

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AU - Saito, Keisuke

AU - Shen, Jian Ren

AU - Ishikita, Hiroshi

N1 - Funding Information: This research was supported by the Japan Science and Technology Precursory Research for Embryonic Science and Technology (JST PRESTO) program (to H.I.), Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (22740276 to K.S.), Special Coordination Fund (to H.I.) for Promoting Science and Technology of MEXT, Takeda Science Foundation (to H.I.), Kyoto University Step-up Grant-in-Aid for young scientists (to H.I.), and Grant for Basic Science Research Projects from The Sumitomo Foundation (to H.I.).

PY - 2012/6/6

Y1 - 2012/6/6

N2 - Influence of the axial ligand of PD1 chlorophyll (D1-His-198) on the Em of monomer chlorophylls PD1 and PD2, and the PD1•+/PD2•+ charge ratio was investigated by theoretical calculations using the PSII crystal structure of Thermosynechococcus vulcanus analyzed at 1.9-Å resolution. It was found that the Em(PD1)/Em(PD2) values and PD1•+/PD2•+ ratio remained unchanged upon D1-H198Q mutation. However, Em(P D1) was increased in the D1-H198A mutant, resulting in a more even distribution of the positive charge over PD1/PD2. Introduction of a water molecule as an axial ligand resulted in equal E m values and PD1•+/PD2 •+ ratios between the mutant and wild-type, thus confirming the presence of the water ligand in the mutant.

AB - Influence of the axial ligand of PD1 chlorophyll (D1-His-198) on the Em of monomer chlorophylls PD1 and PD2, and the PD1•+/PD2•+ charge ratio was investigated by theoretical calculations using the PSII crystal structure of Thermosynechococcus vulcanus analyzed at 1.9-Å resolution. It was found that the Em(PD1)/Em(PD2) values and PD1•+/PD2•+ ratio remained unchanged upon D1-H198Q mutation. However, Em(P D1) was increased in the D1-H198A mutant, resulting in a more even distribution of the positive charge over PD1/PD2. Introduction of a water molecule as an axial ligand resulted in equal E m values and PD1•+/PD2 •+ ratios between the mutant and wild-type, thus confirming the presence of the water ligand in the mutant.

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Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from thermosynechococcus vulcanus

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