Inhibition of plant transformation by phytolaccoside b from phytolacca americana callus

Hiroshi Kanzaki, Toshihiko Kagemori, Yoko Yamachika, Teruhiko Nitoda, Kazuyoshi Kawazu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism.

Original languageEnglish
Pages (from-to)1657-1659
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number9
Publication statusPublished - Jan 1 1999


  • Ageratum conyzoides
  • Agrobacterium tumefaciens
  • GUS expression assay
  • Phytolacca americana

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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