TY - JOUR
T1 - Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha
AU - Nomura, Ryota
AU - Inaba, Hiroaki
AU - Yasuda, Hidemi
AU - Shirai, Mitsuyuki
AU - Kato, Yukio
AU - Murakami, Masaru
AU - Iwashita, Naoki
AU - Shirahata, So
AU - Yoshida, Sho
AU - Matayoshi, Saaya
AU - Yasuda, Junya
AU - Arai, Nobuaki
AU - Asai, Fumitoshi
AU - Matsumoto-Nakano, Michiyo
AU - Nakano, Kazuhiko
N1 - Funding Information:
This study was supported by the Promotion and Mutual Aid Corporation of Private Schools of Japan, and by a Grant-in-Aid for Matching Fund Subsidy for Private Universities.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Porphyromonas gulae is a major periodontal pathogen in dogs, which can be transmitted to their owners. A major virulence factor of P. gulae consists of a 41-kDa filamentous appendage (FimA) on the cell surface, which is classified into three genotypes: A, B, and C. Thus far, inhibition of periodontal disease in dogs remains difficult. The present study assessed the inhibitory effects of a combination of clindamycin and interferon alpha (IFN-α) formulation against P. gulae and periodontal disease. Growth of P. gulae was significantly inhibited by clindamycin; this inhibition had a greater effect on type C P. gulae than on type A and B isolates. In contrast, the IFN-α formulation inhibited the expression of IL-1β and COX-2 elicited by type A and B isolates, but not that elicited by type C isolates. Furthermore, periodontal recovery was promoted by the administration of both clindamycin and IFN-α formulation to dogs undergoing periodontal treatment; moreover, this combined treatment reduced the number of FimA genotypes in oral specimens from treated dogs. These results suggest that a combination of clindamycin and IFN-α formulation inhibit P. gulae virulence and thus may be effective for the prevention of periodontal disease induced by P. gulae.
AB - Porphyromonas gulae is a major periodontal pathogen in dogs, which can be transmitted to their owners. A major virulence factor of P. gulae consists of a 41-kDa filamentous appendage (FimA) on the cell surface, which is classified into three genotypes: A, B, and C. Thus far, inhibition of periodontal disease in dogs remains difficult. The present study assessed the inhibitory effects of a combination of clindamycin and interferon alpha (IFN-α) formulation against P. gulae and periodontal disease. Growth of P. gulae was significantly inhibited by clindamycin; this inhibition had a greater effect on type C P. gulae than on type A and B isolates. In contrast, the IFN-α formulation inhibited the expression of IL-1β and COX-2 elicited by type A and B isolates, but not that elicited by type C isolates. Furthermore, periodontal recovery was promoted by the administration of both clindamycin and IFN-α formulation to dogs undergoing periodontal treatment; moreover, this combined treatment reduced the number of FimA genotypes in oral specimens from treated dogs. These results suggest that a combination of clindamycin and IFN-α formulation inhibit P. gulae virulence and thus may be effective for the prevention of periodontal disease induced by P. gulae.
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U2 - 10.1038/s41598-020-59730-9
DO - 10.1038/s41598-020-59730-9
M3 - Article
C2 - 32080231
AN - SCOPUS:85079816195
SN - 2045-2322
VL - 10
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 3113
ER -