Inhibitory effects of cacao bean husk extract on plaque formation in vitro and in vivo

Michiyo Matsumoto, Masato Tsuji, Jumpei Okuda, Hidekazu Sasaki, Kazuhiko Nakano, Kenji Osawa, Susumu Shimura, Takashi Ooshima

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


Cacao bean husk extract (CBH) has been shown to possess antibacterial and antiglucosyltransferase activities through its unsaturated fatty acids and epicatechin polymers, respectively. In the present study, the antiplaque activities of CBH were examined in vitro and in vivo. The extract inhibited the adherence of Streptococcus mutans MT8148 to saliva-coated hydroxyapatite and reduced the accumulation of artificial dental plaque by S. mutans MT8148 on orthodontic wire. The number of mutans streptococci in dental plaque was also significantly reduced when human dental plaque was exposed to CBH from 21 children at 37°C for 1 h. For the in vivo study, 28 volunteers aged 19-29 yr old rinsed their mouth with CBH, before and after each intake of food and before sleeping at night for 4 d without using other oral hygiene procedures. Plaque depositions and the numbers of mutans streptococci were reduced in the subjects, compared with rinsing with 1 % ethanol alone. These results indicate that CBH possesses significant antiplaque activity in vitro and in vivo.

Original languageEnglish
Pages (from-to)249-252
Number of pages4
JournalEuropean Journal of Oral Sciences
Issue number3
Publication statusPublished - Jun 2004
Externally publishedYes


  • Cacao bean husk
  • Dental plaque
  • Human study
  • Hydroxyapatite
  • Mutans streptococci

ASJC Scopus subject areas

  • Dentistry(all)


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