TY - GEN
T1 - Insulin secreting cell-laden hydrogel microfiber coated with poly-L-ornithine
AU - Watanabe, Takaichi
AU - Okitsu, Teru
AU - Shinohara, Marie
AU - Poudel, Sapana
AU - Sakai, Yasuyuki
AU - Takeuchi, Shoji
N1 - Funding Information:
We acknowledge H. Aoyagi and M. Kiyosawa for their technical assistance. This work was supported by the Research Center Network for Realization of Regenerative Medicine from Japan Science and Technology Agency (JST).
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - This paper describes the preparation of insulin secreting (MIN6m9) cell-laden calcium alginate (Ca-Alg) hydrogel microfiber coated with poly-L-ornithine (PLO) using a microfluidic wet-spinning, followed by layer-by-layer (LbL) deposition to enhance the mechanical strength. Both in vitro and in vivo experiments showed that PLO-coated (PLO-Alg) microfiber had higher mechanical strength than Ca-Alg microfiber. In addition, the cells encapsulated in PLO-Alg microfiber after 7 days of culture secreted insulin in response to glucose concentrations. We believe that PLO-Alg microfiber can be useful for the application in robust tissue graft to achieve long-term implantation.
AB - This paper describes the preparation of insulin secreting (MIN6m9) cell-laden calcium alginate (Ca-Alg) hydrogel microfiber coated with poly-L-ornithine (PLO) using a microfluidic wet-spinning, followed by layer-by-layer (LbL) deposition to enhance the mechanical strength. Both in vitro and in vivo experiments showed that PLO-coated (PLO-Alg) microfiber had higher mechanical strength than Ca-Alg microfiber. In addition, the cells encapsulated in PLO-Alg microfiber after 7 days of culture secreted insulin in response to glucose concentrations. We believe that PLO-Alg microfiber can be useful for the application in robust tissue graft to achieve long-term implantation.
KW - Diabetes mellitus
KW - Hydrogel
KW - Microfiber
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M3 - Conference contribution
AN - SCOPUS:84983247449
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 215
EP - 217
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -
