Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

R. Kawano; Y. Tsuji; M. Hirano; T. Osaki; K. Kamiya; N. Miki; T. Ide; S. Takeuchi

doi:10.1109/MEMSYS.2013.6474175

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, K. Kamiya, N. Miki, T. Ide, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K⁺ channel expressed in nerve system with BLM array were able to be achieved at the high probability.

Original language	English
Title of host publication	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages	57-58
Number of pages	2
DOIs	https://doi.org/10.1109/MEMSYS.2013.6474175
Publication status	Published - 2013
Externally published	Yes
Event	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China Duration: Jan 20 2013 → Jan 24 2013

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Country/Territory	Taiwan, Province of China
City	Taipei
Period	1/20/13 → 1/24/13

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2013.6474175

Cite this

Kawano, R., Tsuji, Y., Hirano, M., Osaki, T., Kamiya, K., Miki, N., Ide, T., & Takeuchi, S. (2013). Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 57-58). Article 6474175 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474175

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. / Kawano, R.; Tsuji, Y.; Hirano, M. et al.
IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 57-58 6474175 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kawano, R, Tsuji, Y, Hirano, M, Osaki, T, Kamiya, K, Miki, N, Ide, T & Takeuchi, S 2013, Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. in IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474175, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 57-58, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 1/20/13. https://doi.org/10.1109/MEMSYS.2013.6474175

Kawano R, Tsuji Y, Hirano M, Osaki T, Kamiya K, Miki N et al. Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 57-58. 6474175. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2013.6474175

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title = "Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level",

abstract = "This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K+ channel expressed in nerve system with BLM array were able to be achieved at the high probability.",

author = "R. Kawano and Y. Tsuji and M. Hirano and T. Osaki and K. Kamiya and N. Miki and T. Ide and S. Takeuchi",

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AU - Kawano, R.

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AU - Ide, T.

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AB - This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K+ channel expressed in nerve system with BLM array were able to be achieved at the high probability.

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Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

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