Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation

Masaaki Tokuda; Bushra Y. Ahmed; Yun Fei Lu; Hideki Matsui; Osamu Miyamoto; Fuminori Yamaguchi; Ryoji Konishi; Osamu Hatase

doi:10.1016/S0006-8993(97)00189-3

Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation

Masaaki Tokuda, Bushra Y. Ahmed, Yun Fei Lu, Hideki Matsui, Osamu Miyamoto, Fuminori Yamaguchi, Ryoji Konishi, Osamu Hatase

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Multifunctional Ca²⁺/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.

Original language	English
Pages (from-to)	162-166
Number of pages	5
Journal	Brain Research
Volume	755
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(97)00189-3
Publication status	Published - Apr 25 1997

Keywords

Ca/calmodulin-dependent protein kinase
Western blot
hippocampus
immunohistochemistry
long-term potentiation

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(97)00189-3

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title = "Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation",

abstract = "Multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.",

keywords = "Ca/calmodulin-dependent protein kinase, Western blot, hippocampus, immunohistochemistry, long-term potentiation",

author = "Masaaki Tokuda and Ahmed, {Bushra Y.} and Lu, {Yun Fei} and Hideki Matsui and Osamu Miyamoto and Fuminori Yamaguchi and Ryoji Konishi and Osamu Hatase",

note = "Funding Information: We are grateful to Dr. Angus C. Nairn (Rockefeller University, USA) and Dr. Hitoshi Fujisawa (Asahikawa University, Japan) for their generous gifts of CaMK-I and -IV antibodies, respectively. We also thank Mr. Keith Philibert (University of Calgary, Canada) for his technical assistance. This work was supported by the grant-in-aid for Scientific Research from The Ministry of Science and Technology, Government of Pakistan and from The Ministry of Education, Science and Culture of Japan.",

year = "1997",

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doi = "10.1016/S0006-8993(97)00189-3",

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T1 - Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation

AU - Tokuda, Masaaki

AU - Ahmed, Bushra Y.

AU - Lu, Yun Fei

AU - Matsui, Hideki

AU - Miyamoto, Osamu

AU - Yamaguchi, Fuminori

AU - Konishi, Ryoji

AU - Hatase, Osamu

N1 - Funding Information: We are grateful to Dr. Angus C. Nairn (Rockefeller University, USA) and Dr. Hitoshi Fujisawa (Asahikawa University, Japan) for their generous gifts of CaMK-I and -IV antibodies, respectively. We also thank Mr. Keith Philibert (University of Calgary, Canada) for his technical assistance. This work was supported by the grant-in-aid for Scientific Research from The Ministry of Science and Technology, Government of Pakistan and from The Ministry of Education, Science and Culture of Japan.

PY - 1997/4/25

Y1 - 1997/4/25

N2 - Multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.

AB - Multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.

KW - Ca/calmodulin-dependent protein kinase

KW - Western blot

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KW - immunohistochemistry

KW - long-term potentiation

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Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation

Abstract

Keywords

ASJC Scopus subject areas

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