Involvement of galectin-9 in guinea pig allergic airway inflammation

Hitomi Yamamoto, Yumiko Kashio, Hiroki Shoji, Rika Shinonaga, Teizo Yoshimura, Nozomu Nishi, Takeshi Nabe, Takanori Nakamura, Shigekatsu Kohno, Mitsuomi Hirashima

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Background: There is little information about the involvement of galectin-9 (Gal-9) in allergic inflammation. Thus, we investigated the role of Gal-9 in asthma model guinea pigs. Methods: Airway resistance (Raw) was measured using a double-flow plethysmograph system. Gal-9 expression in the lung was assessed by Western blot and immunohistochemistry. Eosinophil chemotactic activity was evaluated in a chamber containing a polyvinylpyrolidone-free membrane. Cell apoptosis was analyzed on a flowcytometry with propidium iodide. Results: In cloning guinea pig Gal-9 we identified three isoforms that differ only in the length of their linker peptides, just as with human Gal-9. Guinea pig Gal-9 was found to be a chemoattractant for eosinophils and to promote induction of apoptosis in sensitized but not non-sensitized T lymphocytes. In allergic airway hypersensitivity model, a low level of Gal-9 expression was observed in the nonsensitized/nonchallenged group, but upregulation was detected at 7 h after challenge and sustained up to 24 h. Such upregulation correlated with elevation of eosinophil peroxidase activity but not with increased R aw. Conclusions: The present results provide evidence that Gal-9 is not involved in airway hypersensitivity, but is partly involved in prolonged eosinophil accumulation in the lung.

Original languageEnglish
Pages (from-to)95-105
Number of pages11
JournalInternational archives of allergy and immunology
Issue numberSUPPL. 1
Publication statusPublished - Jun 1 2007
Externally publishedYes


  • Allergic inflammation
  • Animal model
  • Asthma
  • Galectin-9
  • Guinea pig

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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