TY - JOUR
T1 - Involvement of nitric oxide in light-mediated greening of barley seedlings
AU - Zhang, Lingang
AU - Wang, Yading
AU - Zhao, Liqun
AU - Shi, Suyun
AU - Zhang, Lixin
N1 - Funding Information:
This work is supported by Trans-Century Training Programme Foundation for the Talents by the State Education Commission, SRF for ROCS, Key Project of Chinese Ministry of Education.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2006/7/3
Y1 - 2006/7/3
N2 - When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (ΦPSII) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor Nω-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process.
AB - When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (ΦPSII) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor Nω-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process.
KW - Etiolated seedling
KW - Greening
KW - Nitric oxide
KW - Signaling molecule
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U2 - 10.1016/j.jplph.2005.07.011
DO - 10.1016/j.jplph.2005.07.011
M3 - Article
C2 - 16777529
AN - SCOPUS:33744913013
SN - 0176-1617
VL - 163
SP - 818
EP - 826
JO - Z. PFLANZENPHYSIOL.
JF - Z. PFLANZENPHYSIOL.
IS - 8
ER -