Involvement of SA channels in orienting response of cultured endothelial cells to cyclic stretch

Keiji Naruse, Takako Yamada, Masahiro Sokabe

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)


The present work was designed to elucidate the involvement of Ca2+-permeable stretch-activated (SA) channels in the orienting response of endothelial cells to uniaxial cyclic stretch. Endothelial cells from human umbilical vein were cultured on an elastic silicone membrane and subjected to uniaxial cyclic stretch (120% in length, 1 Hz). The cells started to change their morphology 15 min after the onset of stretch, and >90% of the cells oriented perpendicularly to the stretch axis after 2 h. Associated with the orienting response, cell elongation proceeded with a slower rate. Both of the orientating and elongating responses were largely inhibited by the removal of external Ca2+ or by Gd3+, a potent blocker for the SA channel, but not by nifedipine. Intracellular Ca2+ concentration ([Ca2+]i) transiently increased in response to uniaxial stretch, and the basal [Ca2+]i gradually increased during cyclic stretch. This Ca2+ response was inhibited by the removal of extracellular Ca2+ or by the addition of Gd3+. These results suggest that stretch-dependent Ca2+ influx through SA channels is essential in the stretch-dependent cell orientation and elongation.

Original languageEnglish
Pages (from-to)H1532-H1538
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number5
Publication statusPublished - May 1998
Externally publishedYes


  • Calcium mobilization
  • Cell morphology
  • Gadolinium
  • Uniaxial cyclic stretch

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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