Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma

Keiki Ogino, Isao Sakaida, Shinji Oka, Hujio Murakami, Keisuke Hino, Kazutoshi Sanuki, Yasushi Shingai, Hiroshi Nawata, Yuji Kado, Mitsuru Yasunaga, Takaro Ezaki, Keijiro Ando, Kiwamu Okita, Tadayoshi Takemoto

Research output: Contribution to journalArticlepeer-review


Anionic form of glutathione S-transferase (Gst-π)was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21,000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to Ouchtrlony’s method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-n in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson’s grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that Gst-πmay be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.

Original languageEnglish
Pages (from-to)1439-1443
Number of pages5
Issue number10
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Hepatology


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