Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Shigeko Utsugi; Wataru Sakamoto; Yutaka Ogura; Minoru Murata; Fusao Motoyoshi

doi:10.1007/BF00020217

Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Shigeko Utsugi, Wataru Sakamoto, Yutaka Ogura, Minoru Murata, Fusao Motoyoshi

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

Original language	English
Pages (from-to)	759-765
Number of pages	7
Journal	Plant Molecular Biology
Volume	32
Issue number	4
DOIs	https://doi.org/10.1007/BF00020217
Publication status	Published - Jan 1 1996

Keywords

Arabidopsis thaliana
differential screening
flower
transmembrane protein
vegetative storage protein (VSP)

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1007/BF00020217

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title = "Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana",

abstract = "Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.",

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T1 - Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

AU - Utsugi, Shigeko

AU - Sakamoto, Wataru

AU - Ogura, Yutaka

AU - Murata, Minoru

AU - Motoyoshi, Fusao

PY - 1996/1/1

Y1 - 1996/1/1

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AB - Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

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KW - flower

KW - transmembrane protein

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Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Abstract

Keywords

ASJC Scopus subject areas

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