Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

Tomomi Dan; Yuki Mitsui; Hajime Ikeda; Yuji Isagi; Hiroaki Setoguchi

doi:10.1007/s10592-008-9551-z

Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

Tomomi Dan, Yuki Mitsui, Hajime Ikeda, Yuji Isagi, Hiroaki Setoguchi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H _E) and observed (H _O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

Original language	English
Pages (from-to)	507-509
Number of pages	3
Journal	Conservation Genetics
Volume	10
Issue number	3
DOIs	https://doi.org/10.1007/s10592-008-9551-z
Publication status	Published - Jun 1 2009
Externally published	Yes

Keywords

Conservation genetics
Heterozygosity
Microsatellite
Ryukyu Islands
Shortia rotundifolia

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s10592-008-9551-z

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abstract = "Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.",

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T1 - Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

AU - Dan, Tomomi

AU - Mitsui, Yuki

AU - Ikeda, Hajime

AU - Isagi, Yuji

AU - Setoguchi, Hiroaki

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

AB - Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

KW - Conservation genetics

KW - Heterozygosity

KW - Microsatellite

KW - Ryukyu Islands

KW - Shortia rotundifolia

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DO - 10.1007/s10592-008-9551-z

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Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

Abstract

Keywords

ASJC Scopus subject areas

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