Isolation and characterization of the nuclei from Saccharomyces cerevisiae

Kazuo Kamimura; Eiko Tsuchiya; Tokichi Miyakawa; Sakuzo Fuki; Aiko Hirata

doi:10.1007/BF01642394

Isolation and characterization of the nuclei from Saccharomyces cerevisiae

Kazuo Kamimura, Eiko Tsuchiya, Tokichi Miyakawa, Sakuzo Fuki, Aiko Hirata

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Current Microbiology
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/BF01642394
Publication status	Published - May 1981
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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10.1007/BF01642394

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title = "Isolation and characterization of the nuclei from Saccharomyces cerevisiae",

abstract = "We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.",

author = "Kazuo Kamimura and Eiko Tsuchiya and Tokichi Miyakawa and Sakuzo Fuki and Aiko Hirata",

year = "1981",

month = may,

doi = "10.1007/BF01642394",

language = "English",

volume = "6",

pages = "175--180",

journal = "Current Microbiology",

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T1 - Isolation and characterization of the nuclei from Saccharomyces cerevisiae

AU - Kamimura, Kazuo

AU - Tsuchiya, Eiko

AU - Miyakawa, Tokichi

AU - Fuki, Sakuzo

AU - Hirata, Aiko

PY - 1981/5

Y1 - 1981/5

N2 - We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

AB - We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

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U2 - 10.1007/BF01642394

DO - 10.1007/BF01642394

M3 - Article

AN - SCOPUS:0019814904

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VL - 6

SP - 175

EP - 180

JO - Current Microbiology

JF - Current Microbiology

IS - 3

ER -

Isolation and characterization of the nuclei from Saccharomyces cerevisiae

Abstract

ASJC Scopus subject areas

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