Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate

Xiaoyang Liu; Zixu Wang; Jing Zhang; Liang Song; Deyang Li; Zixuan Wu; Beiwei Zhu; Yoshimasa Nakamura; Fereidoon Shahidi; Chenxu Yu; Dayong Zhou

doi:10.1002/jsfa.9919

Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate

Xiaoyang Liu, Zixu Wang, Jing Zhang, Liang Song, Deyang Li, Zixuan Wu, Beiwei Zhu, Yoshimasa Nakamura, Fereidoon Shahidi, Chenxu Yu, Dayong Zhou

School of Agriculture

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

BACKGROUND: Zinc is known to play an essential role in the biological activities in the human body. In this study, a zinc-chelating peptide (ZCP) produced by Alcalase-assisted hydrolysis of the body wall of sea cucumber was isolated and identified. The ZCP was purified stepwise by ultrafiltration, anion-exchange chromatography, and gel filtration chromatography, in conjunction with ultraviolet–visual (UV–visual) spectrophotometry, which was used to analyze each purified fraction. RESULTS: Analysis of the purified ZCP revealed that its zinc-chelating ability was 33.31%. Analysis of isothermal titration calorimetry suggested that the binding of ZCP and zinc (N ≈ 2) was endothermic, with weak binding affinity. Fourier transform infrared spectroscopy spectra (FTIR) indicated that carboxylic and amide groups in ZCP were the primary binding sites of Zn. Sequencing the result by ultra-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) showed that a representative ZCP had the sequence WLTPTYPE with a molecular weight of 1005.5 Da. CONCLUSION: These results provide a promising foundation for the production of zinc supplements from sea-cucumber-derived ZCPs.

Original language	English
Pages (from-to)	6400-6407
Number of pages	8
Journal	Journal of the Science of Food and Agriculture
Volume	99
Issue number	14
DOIs	https://doi.org/10.1002/jsfa.9919
Publication status	Published - Nov 1 2019

Keywords

hydrolysate
peptide sequencing
purification
sea cucumber
zinc-chelating peptides

ASJC Scopus subject areas

Biotechnology
Food Science
Agronomy and Crop Science
Nutrition and Dietetics

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10.1002/jsfa.9919

Cite this

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title = "Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate",

abstract = "BACKGROUND: Zinc is known to play an essential role in the biological activities in the human body. In this study, a zinc-chelating peptide (ZCP) produced by Alcalase-assisted hydrolysis of the body wall of sea cucumber was isolated and identified. The ZCP was purified stepwise by ultrafiltration, anion-exchange chromatography, and gel filtration chromatography, in conjunction with ultraviolet–visual (UV–visual) spectrophotometry, which was used to analyze each purified fraction. RESULTS: Analysis of the purified ZCP revealed that its zinc-chelating ability was 33.31%. Analysis of isothermal titration calorimetry suggested that the binding of ZCP and zinc (N ≈ 2) was endothermic, with weak binding affinity. Fourier transform infrared spectroscopy spectra (FTIR) indicated that carboxylic and amide groups in ZCP were the primary binding sites of Zn. Sequencing the result by ultra-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) showed that a representative ZCP had the sequence WLTPTYPE with a molecular weight of 1005.5 Da. CONCLUSION: These results provide a promising foundation for the production of zinc supplements from sea-cucumber-derived ZCPs.",

keywords = "hydrolysate, peptide sequencing, purification, sea cucumber, zinc-chelating peptides",

author = "Xiaoyang Liu and Zixu Wang and Jing Zhang and Liang Song and Deyang Li and Zixuan Wu and Beiwei Zhu and Yoshimasa Nakamura and Fereidoon Shahidi and Chenxu Yu and Dayong Zhou",

note = "Funding Information: This work was supported financially by the National Natural Science Foundation of China (31801554).",

year = "2019",

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doi = "10.1002/jsfa.9919",

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T1 - Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate

AU - Liu, Xiaoyang

AU - Wang, Zixu

AU - Zhang, Jing

AU - Song, Liang

AU - Li, Deyang

AU - Wu, Zixuan

AU - Zhu, Beiwei

AU - Nakamura, Yoshimasa

AU - Shahidi, Fereidoon

AU - Yu, Chenxu

AU - Zhou, Dayong

N1 - Funding Information: This work was supported financially by the National Natural Science Foundation of China (31801554).

PY - 2019/11/1

Y1 - 2019/11/1

N2 - BACKGROUND: Zinc is known to play an essential role in the biological activities in the human body. In this study, a zinc-chelating peptide (ZCP) produced by Alcalase-assisted hydrolysis of the body wall of sea cucumber was isolated and identified. The ZCP was purified stepwise by ultrafiltration, anion-exchange chromatography, and gel filtration chromatography, in conjunction with ultraviolet–visual (UV–visual) spectrophotometry, which was used to analyze each purified fraction. RESULTS: Analysis of the purified ZCP revealed that its zinc-chelating ability was 33.31%. Analysis of isothermal titration calorimetry suggested that the binding of ZCP and zinc (N ≈ 2) was endothermic, with weak binding affinity. Fourier transform infrared spectroscopy spectra (FTIR) indicated that carboxylic and amide groups in ZCP were the primary binding sites of Zn. Sequencing the result by ultra-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) showed that a representative ZCP had the sequence WLTPTYPE with a molecular weight of 1005.5 Da. CONCLUSION: These results provide a promising foundation for the production of zinc supplements from sea-cucumber-derived ZCPs.

AB - BACKGROUND: Zinc is known to play an essential role in the biological activities in the human body. In this study, a zinc-chelating peptide (ZCP) produced by Alcalase-assisted hydrolysis of the body wall of sea cucumber was isolated and identified. The ZCP was purified stepwise by ultrafiltration, anion-exchange chromatography, and gel filtration chromatography, in conjunction with ultraviolet–visual (UV–visual) spectrophotometry, which was used to analyze each purified fraction. RESULTS: Analysis of the purified ZCP revealed that its zinc-chelating ability was 33.31%. Analysis of isothermal titration calorimetry suggested that the binding of ZCP and zinc (N ≈ 2) was endothermic, with weak binding affinity. Fourier transform infrared spectroscopy spectra (FTIR) indicated that carboxylic and amide groups in ZCP were the primary binding sites of Zn. Sequencing the result by ultra-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) showed that a representative ZCP had the sequence WLTPTYPE with a molecular weight of 1005.5 Da. CONCLUSION: These results provide a promising foundation for the production of zinc supplements from sea-cucumber-derived ZCPs.

KW - hydrolysate

KW - peptide sequencing

KW - purification

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KW - zinc-chelating peptides

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Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate

Abstract

Keywords

ASJC Scopus subject areas

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