Isolation of a New Minor Protein (Ovofactor-1), Which Has a Cell Growth Promoting Activity, from Hen's Egg White by Heparin Affinity Chromatography

Tadashi Nakamura; Tadao Saito; Haruki Kitazawa; Sanae Takeuchi; Takatoshi Itoh

doi:10.1271/bbb.59.1946

Isolation of a New Minor Protein (Ovofactor-1), Which Has a Cell Growth Promoting Activity, from Hen's Egg White by Heparin Affinity Chromatography

Tadashi Nakamura, Tadao Saito, Haruki Kitazawa, Sanae Takeuchi, Takatoshi Itoh

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A proteinaceous component eluted at the concentration of 0.8-1.0 M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS-PAGE, the component was resolved into three bands of 18. 5, 16. 5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chichen embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named “Ovofactor-1”.

Original language	English
Pages (from-to)	1946-1948
Number of pages	3
Journal	Bioscience, biotechnology, and biochemistry
Volume	59
Issue number	10
DOIs	https://doi.org/10.1271/bbb.59.1946
Publication status	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "A proteinaceous component eluted at the concentration of 0.8-1.0 M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS-PAGE, the component was resolved into three bands of 18. 5, 16. 5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chichen embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named “Ovofactor-1”.",

author = "Tadashi Nakamura and Tadao Saito and Haruki Kitazawa and Sanae Takeuchi and Takatoshi Itoh",

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AU - Saito, Tadao

AU - Kitazawa, Haruki

AU - Takeuchi, Sanae

AU - Itoh, Takatoshi

PY - 1995

Y1 - 1995

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JO - Bioscience, biotechnology, and biochemistry

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ER -

Isolation of a New Minor Protein (Ovofactor-1), Which Has a Cell Growth Promoting Activity, from Hen's Egg White by Heparin Affinity Chromatography

Abstract

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