Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method

We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5,-9 and -19. The expression of these genes was slightly stimulated lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rabl6A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lipl9 genes were induced 6 h after such a shift.