Isolation of small RNAs using biotinylated PNAs

Takashi Ohtsuki; Takeshi Fujimoto; Maya Kamimukai; Chisato Kumano; Mizuki Kitamatsu; Masahiko Sisido

doi:10.1093/jb/mvn107

Isolation of small RNAs using biotinylated PNAs

Takashi Ohtsuki, Takeshi Fujimoto, Maya Kamimukai, Chisato Kumano, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.

Original language	English
Pages (from-to)	415-418
Number of pages	4
Journal	Journal of biochemistry
Volume	144
Issue number	4
DOIs	https://doi.org/10.1093/jb/mvn107
Publication status	Published - Oct 2008

Keywords

Non-coding RNA
Peptide nucleic acid
RNA isolation
Ribosomal RNA
Transfer RNA

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1093/jb/mvn107

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abstract = "In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.",

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author = "Takashi Ohtsuki and Takeshi Fujimoto and Maya Kamimukai and Chisato Kumano and Mizuki Kitamatsu and Masahiko Sisido",

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volume = "144",

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T1 - Isolation of small RNAs using biotinylated PNAs

AU - Ohtsuki, Takashi

AU - Fujimoto, Takeshi

AU - Kamimukai, Maya

AU - Kumano, Chisato

AU - Kitamatsu, Mizuki

AU - Sisido, Masahiko

PY - 2008/10

Y1 - 2008/10

N2 - In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.

AB - In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.

KW - Non-coding RNA

KW - Peptide nucleic acid

KW - RNA isolation

KW - Ribosomal RNA

KW - Transfer RNA

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EP - 418

JO - Journal of Biochemistry

JF - Journal of Biochemistry

IS - 4

ER -

Isolation of small RNAs using biotinylated PNAs

Abstract

Keywords

ASJC Scopus subject areas

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