Izuchenie protsessa vydeleniia kisloroda v khloroplastakh gorokha metodom spinovykh metok.

A. V. Kulikov; E. I. Iudanova; G. I. Likhtensteǐn; S. I. Allakhverdiev; V. V. Klimov

Izuchenie protsessa vydeleniia kisloroda v khloroplastakh gorokha metodom spinovykh metok.

Translated title of the contribution: [Study of oxygen accumulation in pea chloroplasts with spin labels].

A. V. Kulikov, E. I. Iudanova, G. I. Likhtensteǐn, S. I. Allakhverdiev, V. V. Klimov

Research output: Contribution to journal › Article › peer-review

Abstract

Oxygen evolution in chloroplasts was studied by nitroxide fatty probes, introduced into chloroplasts membranes. The values of K(e)[O ²] were determined from the measuring kinetics of nitroxide reduction under permanent illumination at two values of the microwave field, where K(e) was the constant of spin exchange between nitroxide and oxygen, [O ²] --oxygen concentration. It was shown that in chloroplasts membranes, in contrast to liposomes there was no oxygen in the dark. This observation can be explained by oxygen consumption in various biochemical reactions. The values of K(e)[O ²] were measured under permanent illumination. The highest value of K(e)[O ²]=1.2.10(-5) s(-2) was observed in the middle of the membrane. At temperatures above 40 ⁰C and below -20 ⁰C oxygen was not evolved.

Translated title of the contribution	[Study of oxygen accumulation in pea chloroplasts with spin labels].
Original language	Russian
Pages (from-to)	984-989
Number of pages	6
Journal	Biofizika
Volume	33
Issue number	6
Publication status	Published - Nov 1 1988

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "Izuchenie protsessa vydeleniia kisloroda v khloroplastakh gorokha metodom spinovykh metok.",

abstract = "Oxygen evolution in chloroplasts was studied by nitroxide fatty probes, introduced into chloroplasts membranes. The values of K(e)[O 2] were determined from the measuring kinetics of nitroxide reduction under permanent illumination at two values of the microwave field, where K(e) was the constant of spin exchange between nitroxide and oxygen, [O 2] --oxygen concentration. It was shown that in chloroplasts membranes, in contrast to liposomes there was no oxygen in the dark. This observation can be explained by oxygen consumption in various biochemical reactions. The values of K(e)[O 2] were measured under permanent illumination. The highest value of K(e)[O 2]=1.2.10(-5) s(-2) was observed in the middle of the membrane. At temperatures above 40 0C and below -20 0C oxygen was not evolved.",

author = "Kulikov, {A. V.} and Iudanova, {E. I.} and Likhtensteǐn, {G. I.} and Allakhverdiev, {S. I.} and Klimov, {V. V.}",

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T1 - Izuchenie protsessa vydeleniia kisloroda v khloroplastakh gorokha metodom spinovykh metok.

AU - Kulikov, A. V.

AU - Iudanova, E. I.

AU - Likhtensteǐn, G. I.

AU - Allakhverdiev, S. I.

AU - Klimov, V. V.

PY - 1988/11/1

Y1 - 1988/11/1

N2 - Oxygen evolution in chloroplasts was studied by nitroxide fatty probes, introduced into chloroplasts membranes. The values of K(e)[O 2] were determined from the measuring kinetics of nitroxide reduction under permanent illumination at two values of the microwave field, where K(e) was the constant of spin exchange between nitroxide and oxygen, [O 2] --oxygen concentration. It was shown that in chloroplasts membranes, in contrast to liposomes there was no oxygen in the dark. This observation can be explained by oxygen consumption in various biochemical reactions. The values of K(e)[O 2] were measured under permanent illumination. The highest value of K(e)[O 2]=1.2.10(-5) s(-2) was observed in the middle of the membrane. At temperatures above 40 0C and below -20 0C oxygen was not evolved.

AB - Oxygen evolution in chloroplasts was studied by nitroxide fatty probes, introduced into chloroplasts membranes. The values of K(e)[O 2] were determined from the measuring kinetics of nitroxide reduction under permanent illumination at two values of the microwave field, where K(e) was the constant of spin exchange between nitroxide and oxygen, [O 2] --oxygen concentration. It was shown that in chloroplasts membranes, in contrast to liposomes there was no oxygen in the dark. This observation can be explained by oxygen consumption in various biochemical reactions. The values of K(e)[O 2] were measured under permanent illumination. The highest value of K(e)[O 2]=1.2.10(-5) s(-2) was observed in the middle of the membrane. At temperatures above 40 0C and below -20 0C oxygen was not evolved.

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Izuchenie protsessa vydeleniia kisloroda v khloroplastakh gorokha metodom spinovykh metok.

Abstract

ASJC Scopus subject areas

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