TY - CHAP
T1 - Karyotyping methods for fungi
AU - Mehrabi, Rahim
AU - Taga, Masatoki
AU - Aghaee, Mostafa
AU - De Wit, Pierre J.G.M.
AU - Kema, Gert H.J.
PY - 2012
Y1 - 2012
N2 - Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.
AB - Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.
KW - Chromosome polymorphisms
KW - Electrophoretic karyotyping
KW - Germ tube burst method
KW - Pulsed field gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=84855895463&partnerID=8YFLogxK
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U2 - 10.1007/978-1-61779-501-5_37
DO - 10.1007/978-1-61779-501-5_37
M3 - Chapter
C2 - 22183680
AN - SCOPUS:84855895463
SN - 9781617795008
T3 - Methods in Molecular Biology
SP - 591
EP - 602
BT - Plant Fungal Pathogens
ER -