Karyotyping methods for fungi

Rahim Mehrabi, Masatoki Taga, Mostafa Aghaee, Pierre J.G.M. De Wit, Gert H.J. Kema

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)


Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.

Original languageEnglish
Title of host publicationPlant Fungal Pathogens
Subtitle of host publicationMethods and Protocols
Number of pages12
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Chromosome polymorphisms
  • Electrophoretic karyotyping
  • Germ tube burst method
  • Pulsed field gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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