KmrA multidrug efflux pump from Klebsiella pneumoniae

Wakano Ogawa; Motohiro Koterasawa; Teruo Kuroda; Tomofusa Tsuchiya

doi:10.1248/bpb.29.550

KmrA multidrug efflux pump from Klebsiella pneumoniae

Wakano Ogawa, Motohiro Koterasawa, Teruo Kuroda, Tomofusa Tsuchiya

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We cloned a gene responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 that showed multidrug resistance. We designated the gene kmrA. The deduced amino acid sequence of KmrA was similar to that of SmvA that is responsible for methyl viologen-resistance in Salmonella enterica sv. Typhi and Typhimurium. Introduction of the cloned kmrA gene into drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst 33342, tetraphenylphosphonium chloride (TPPCl), methyl viologen and ethidium bromide. We observed elevated energy-dependent efflux of ethidium in E. coli cells carrying the kmrA gene compared with control cells. We also cloned the smvA gene from S. enterica sv. Typhimurium LT2 and investigated the resistance pattern for several drugs. The pattern was similar between KmrA from K. pneumoniae and SmvA from S. enterica.

Original language	English
Pages (from-to)	550-553
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	29
Issue number	3
DOIs	https://doi.org/10.1248/bpb.29.550
Publication status	Published - Mar 2006

Keywords

Klebsiella pneumoniae
KmrA
Multidrug efflux pump
Salmonella enterica
SmvA

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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10.1248/bpb.29.550

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title = "KmrA multidrug efflux pump from Klebsiella pneumoniae",

abstract = "We cloned a gene responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 that showed multidrug resistance. We designated the gene kmrA. The deduced amino acid sequence of KmrA was similar to that of SmvA that is responsible for methyl viologen-resistance in Salmonella enterica sv. Typhi and Typhimurium. Introduction of the cloned kmrA gene into drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst 33342, tetraphenylphosphonium chloride (TPPCl), methyl viologen and ethidium bromide. We observed elevated energy-dependent efflux of ethidium in E. coli cells carrying the kmrA gene compared with control cells. We also cloned the smvA gene from S. enterica sv. Typhimurium LT2 and investigated the resistance pattern for several drugs. The pattern was similar between KmrA from K. pneumoniae and SmvA from S. enterica.",

keywords = "Klebsiella pneumoniae, KmrA, Multidrug efflux pump, Salmonella enterica, SmvA",

author = "Wakano Ogawa and Motohiro Koterasawa and Teruo Kuroda and Tomofusa Tsuchiya",

year = "2006",

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doi = "10.1248/bpb.29.550",

language = "English",

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AU - Ogawa, Wakano

AU - Koterasawa, Motohiro

AU - Kuroda, Teruo

AU - Tsuchiya, Tomofusa

PY - 2006/3

Y1 - 2006/3

N2 - We cloned a gene responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 that showed multidrug resistance. We designated the gene kmrA. The deduced amino acid sequence of KmrA was similar to that of SmvA that is responsible for methyl viologen-resistance in Salmonella enterica sv. Typhi and Typhimurium. Introduction of the cloned kmrA gene into drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst 33342, tetraphenylphosphonium chloride (TPPCl), methyl viologen and ethidium bromide. We observed elevated energy-dependent efflux of ethidium in E. coli cells carrying the kmrA gene compared with control cells. We also cloned the smvA gene from S. enterica sv. Typhimurium LT2 and investigated the resistance pattern for several drugs. The pattern was similar between KmrA from K. pneumoniae and SmvA from S. enterica.

AB - We cloned a gene responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 that showed multidrug resistance. We designated the gene kmrA. The deduced amino acid sequence of KmrA was similar to that of SmvA that is responsible for methyl viologen-resistance in Salmonella enterica sv. Typhi and Typhimurium. Introduction of the cloned kmrA gene into drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst 33342, tetraphenylphosphonium chloride (TPPCl), methyl viologen and ethidium bromide. We observed elevated energy-dependent efflux of ethidium in E. coli cells carrying the kmrA gene compared with control cells. We also cloned the smvA gene from S. enterica sv. Typhimurium LT2 and investigated the resistance pattern for several drugs. The pattern was similar between KmrA from K. pneumoniae and SmvA from S. enterica.

KW - Klebsiella pneumoniae

KW - KmrA

KW - Multidrug efflux pump

KW - Salmonella enterica

KW - SmvA

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KmrA multidrug efflux pump from Klebsiella pneumoniae

Abstract

Keywords

ASJC Scopus subject areas

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