TY - JOUR
T1 - Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
AU - Miyagi-Shiohira, Chika
AU - Saitoh, Issei
AU - Watanabe, Masami
AU - Noguchi, Hirofumi
N1 - Funding Information:
We thank Naomi Kakazu (University of the Ryukyus) for administration and Maki Higa, Yuki Kawahira, and Ikue Honda (University of the Ryukyus) for technical support. This work was supported in part by JSPS KAKENHI Grant Numbers 19K09051, 17H00769, 19H01064, and 17H04412, and the Okinawa Science and Technology Innovation System Construction Project.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Kyoto probe 1 (KP-1) rapidly distinguishes between human ES/iPS (hES/iPS) cells and their differentiated cells. Recently, we generated induced tissue-specific stem cells from pancreas (iTS-P cells) using reprogramming factors and tissue-specific selection. The iTS-P cells have self-renewal potential, and subcutaneously transplanting them into immunodeficient mice did not generate teratomas. In this study, we applied KP-1 to analyze mouse ES (mES) cells and mouse iTS-P (miTS-P) cells. KP-1 completely stained mES cells in colonies, but only miTS-P cells at the edge of a colony. This difference was caused by cell type-specific expression of different ABC transporters. These finding suggest that KP-1 will be useful for distinguishing between iPS and iTS-P cells.
AB - Kyoto probe 1 (KP-1) rapidly distinguishes between human ES/iPS (hES/iPS) cells and their differentiated cells. Recently, we generated induced tissue-specific stem cells from pancreas (iTS-P cells) using reprogramming factors and tissue-specific selection. The iTS-P cells have self-renewal potential, and subcutaneously transplanting them into immunodeficient mice did not generate teratomas. In this study, we applied KP-1 to analyze mouse ES (mES) cells and mouse iTS-P (miTS-P) cells. KP-1 completely stained mES cells in colonies, but only miTS-P cells at the edge of a colony. This difference was caused by cell type-specific expression of different ABC transporters. These finding suggest that KP-1 will be useful for distinguishing between iPS and iTS-P cells.
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U2 - 10.1038/s41598-020-75016-6
DO - 10.1038/s41598-020-75016-6
M3 - Article
C2 - 33093580
AN - SCOPUS:85093534152
SN - 2045-2322
VL - 10
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 18084
ER -