L-selectin and its ligands mediate infiltration of mononuclear cells into kidney interstitium after ureteric obstruction

Kenichi Shikata, Yasuo Suzuki, Jun Wada, Kyoji Hirata, Mitsuhiro Matsuda, Hiroto Kawashima, Takashi Suzuki, Masako Iizuka, Hirofumi Makino, Masayuki Miyasaka

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38 Citations (Scopus)


It was previously reported that the L-selectin ligands detected by a rat L-selectin and human IgG chimeric molecule (rLEC-IgG) are expressed in the distal tubules of the kidney, where no leukocyte traffic is seen under physiological conditions. In the present study, the role of L-selectin ligands in leukocyte infiltration into the kidney interstitium was investigated using a rat ureteric obstruction model. After ligation of the ureter, ligands for L-selectin rapidly disappeared from tubular epithelial cells and were relocated to the interstitium and peritubular capillary walls, where infiltration of monocytes and CD8+ T cells subsequently occurred. Mononuclear cell infiltration was significantly inhibited by intravenous injection of a neutralizing monoclonal antibody (MAb) against L-selectin, indicating the possible involvement of an L-selectin-mediated pathway. Interestingly, immunohistochemical studies with a MAb against sulphatide showed that the distribution of sulphatide, known to be one of the candidates of L-selectin ligand, was almost indistinguishable from the staining pattern of rLEC-IgG in both normal and ureteric obstructed kidneys, suggesting that sulphatide and/or related molecule(s) relocated to the renal interstitium were recognized by leukocyte L-selectin, leading to interstitial leukocyte infiltration. In line with this notion, intravenous injection of sulphatide markedly inhibited leukocyte infiltration, suggesting that L-selectin- sulphatide interaction may play a pivotal role in interstitial leukocyte infiltration in the kidney following ureteric obstruction.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalJournal of Pathology
Issue number1
Publication statusPublished - 1999


  • Inflammation
  • L-selectin
  • Obstructive nephropathy
  • Sulphatide

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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