TY - JOUR
T1 - Label-free detection of low-molecular-weight samples using a terahertz chemical microscope
AU - Kuwana, Takuya
AU - Ogawa, Masahiro
AU - Sakai, Kenji
AU - Kiwa, Toshihiko
AU - Tsukada, Keiji
N1 - Publisher Copyright:
© 2016 The Japan Society of Applied Physics.
PY - 2016/3/14
Y1 - 2016/3/14
N2 - A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of biomolecular interactions on a sensing plate without any labels. In this study, the concanavalin A (Con A)-D-(+)-mannose (mannose) interaction was detected using the TCM with mannose applied as the analyte and Con A immobilized on the sensing plate. To demonstrate this interaction, the amplitude of terahertz pulses as a function of Con A-mannose interaction time, as well as the Con A-mannose coupling concentration, was evaluated. The results suggest that coupling kinetics may be evaluated using a TCM.
AB - A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of biomolecular interactions on a sensing plate without any labels. In this study, the concanavalin A (Con A)-D-(+)-mannose (mannose) interaction was detected using the TCM with mannose applied as the analyte and Con A immobilized on the sensing plate. To demonstrate this interaction, the amplitude of terahertz pulses as a function of Con A-mannose interaction time, as well as the Con A-mannose coupling concentration, was evaluated. The results suggest that coupling kinetics may be evaluated using a TCM.
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U2 - 10.7567/APEX.9.042401
DO - 10.7567/APEX.9.042401
M3 - Article
AN - SCOPUS:84963563123
SN - 1882-0778
VL - 9
SP - 42401
JO - Applied Physics Express
JF - Applied Physics Express
IS - 4
M1 - 42401
ER -