TY - GEN
T1 - Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media
AU - Moniruzzaman, Muhammad
AU - Nao, Yoichiro
AU - Bhattacharjee, Sekhar
AU - Ono, Tsutomu
N1 - Publisher Copyright:
© 2014 Trans Tech Publications, Switzerland.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) [C2mim][PF6] (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions.
AB - Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) [C2mim][PF6] (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions.
KW - Enzyme activity and stability
KW - Enzyme immobilization
KW - Ionic liquids
KW - Laccase poly (ethylene glycol)-block-polylactide emulsions
UR - http://www.scopus.com/inward/record.url?scp=84914177548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84914177548&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMM.625.333
DO - 10.4028/www.scientific.net/AMM.625.333
M3 - Conference contribution
AN - SCOPUS:84914177548
T3 - Applied Mechanics and Materials
SP - 333
EP - 336
BT - Process and Advanced Materials Engineering
A2 - Ahmed, Iqbal
PB - Trans Tech Publications Ltd
T2 - 3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014
Y2 - 3 June 2014 through 5 June 2014
ER -