Lack of occlusal support did not impact amyloid β deposition in APP knock-in mice

Asuka Murakami, Tetsuya Hara, Chie Yamada-Kubota, Miho Kuwahara, Tomoka Ichikawa, Shogo Minagi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Purpose: The lack of occlusal support is an epidemiological risk factor linked to Alzheimer’s disease. This study sought to assess the relationship between amyloid β (Aβ) deposition and the lack of occlusal support in amyloid precursor protein (APP) knock-in mice. Methods: Sixteen experimental animals were divided into two groups. The upper molars were extracted in the extraction group (group E), and a sham operation was performed in the control group (group C). The Morris water maze test was performed 4 months after the tooth extraction. Aβ immunohistochemical staining and Nissl staining of the hippocampus were performed. Hippocampal plasma corticosterone and Aβ protein levels were measured. Results: In the maze task, the escape latency was significantly longer in group E than in group C. In the probe trials, the time elapsed in the target quadrant was significantly shorter in group E than in group C. The number of hippocampal neurons decreased in group E. There was no significant difference in the plasma corticosterone levels between the two groups, indicating that there was no effect of chronic stress on the behavioral results. Hippocampal Aβ40 and Aβ42 protein levels and Aβ deposition areas by immunohistochemical staining were not significantly different between the two groups. Conclusion: Aβ deposition was not increased in the hippocampus of molarless APP knock-in mice. As such, it appears that cognitive impairment due to a lack of occlusal support was not related to Aβ deposition.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of Prosthodontic Research
Issue number1
Publication statusPublished - 2022


  • Amyloid β deposition
  • APP knock-in mice
  • Lack of occlusal support

ASJC Scopus subject areas

  • Oral Surgery
  • Dentistry (miscellaneous)


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