TY - JOUR
T1 - Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice
AU - Nakamura, Ichiro
AU - Takahashi, Naoyuki
AU - Udagawa, Nobuyuki
AU - Moriyama, Yoshinori
AU - Kurokawa, Takahide
AU - Jimi, Eijiro
AU - Sasaki, Takahisa
AU - Suda, Tatsuo
N1 - Funding Information:
We thank Dr. Ryu-ichi Nishinakamura (University of Tokyo) for his valuable advice, and Dr. H. Kalervo Väänänen for the gift of anti-carbonic anhydrase II antibody. This study was supported in part by Grants-in-Aid (08672088, 07557118 and 07457441) from the Ministry of Science, Education and Culture of Japan.
PY - 1997/1/20
Y1 - 1997/1/20
N2 - We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+-ATPase (V-ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V-ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell-derived osteoclast-like cells (OCLs), immunoreactivity for V-ATPase was detected in association with Triton X-100-insoluble cellular structure, but not in oc/oc spleen cell-derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V-ATPase and cytoskeleton in osteoclasts.
AB - We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+-ATPase (V-ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V-ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell-derived osteoclast-like cells (OCLs), immunoreactivity for V-ATPase was detected in association with Triton X-100-insoluble cellular structure, but not in oc/oc spleen cell-derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V-ATPase and cytoskeleton in osteoclasts.
KW - Osteosclerosis
KW - Renal proximal convoluted tubule
KW - Ruffled border
KW - Vacuolar proton ATPase
KW - oc/oc
UR - http://www.scopus.com/inward/record.url?scp=0031015595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031015595&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(96)01454-8
DO - 10.1016/S0014-5793(96)01454-8
M3 - Article
C2 - 9013888
AN - SCOPUS:0031015595
SN - 0014-5793
VL - 401
SP - 207
EP - 212
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -