Large conductance Ca2+-activated K+ channels inhibit vagal acetylcholine release at the rabbit sinoatrial node

Toru Kawada; Tsuyoshi Akiyama; Shuji Shimizu; Atsunori Kamiya; Kazunori Uemura; Yusuke Sata; Mikiyasu Shirai; Masaru Sugimachi

doi:10.1016/j.autneu.2010.04.003

Large conductance Ca²⁺-activated K⁺ channels inhibit vagal acetylcholine release at the rabbit sinoatrial node

Toru Kawada, Tsuyoshi Akiyama, Shuji Shimizu, Atsunori Kamiya, Kazunori Uemura, Yusuke Sata, Mikiyasu Shirai, Masaru Sugimachi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Although large conductance Ca²⁺-activated K⁺ (BK) channels play an important role in determining vascular tone, their role in the efferent cardiac vagal system remains to be elucidated. In anesthetized rabbits (n=9), acetylcholine (ACh) was measured at the right atrium near the sinoatrial node by a cardiac microdialysis technique, and the ACh release in response to electrical stimulation of the cervical preganglionic vagal nerves was examined. Local administration of a BK channel blocker iberiotoxin (2μM) through a dialysis fiber increased the stimulation-induced ACh release from 7.6±2.7 to 9.0±3.2nM (P<0.05). Addition of intravenous administration of iberiotoxin (0.11mg/body) did not increase the stimulation-induced ACh release further (10.8±4.4nM). These results indicate that the BK channels play an inhibitory role in the vagal ACh release to the sinoatrial node.

Original language	English
Pages (from-to)	149-151
Number of pages	3
Journal	Autonomic Neuroscience: Basic and Clinical
Volume	156
Issue number	1-2
DOIs	https://doi.org/10.1016/j.autneu.2010.04.003
Publication status	Published - Aug 2010
Externally published	Yes

Keywords

Acetylcholine
Cardiac microdialysis
Rabbits
Vagal stimulation

ASJC Scopus subject areas

Endocrine and Autonomic Systems
Clinical Neurology
Cellular and Molecular Neuroscience

Access to Document

10.1016/j.autneu.2010.04.003

Cite this

@article{fafe73488184475bbe66f892ef1ce1c4,

title = "Large conductance Ca2+-activated K+ channels inhibit vagal acetylcholine release at the rabbit sinoatrial node",

abstract = "Although large conductance Ca2+-activated K+ (BK) channels play an important role in determining vascular tone, their role in the efferent cardiac vagal system remains to be elucidated. In anesthetized rabbits (n=9), acetylcholine (ACh) was measured at the right atrium near the sinoatrial node by a cardiac microdialysis technique, and the ACh release in response to electrical stimulation of the cervical preganglionic vagal nerves was examined. Local administration of a BK channel blocker iberiotoxin (2μM) through a dialysis fiber increased the stimulation-induced ACh release from 7.6±2.7 to 9.0±3.2nM (P<0.05). Addition of intravenous administration of iberiotoxin (0.11mg/body) did not increase the stimulation-induced ACh release further (10.8±4.4nM). These results indicate that the BK channels play an inhibitory role in the vagal ACh release to the sinoatrial node.",

keywords = "Acetylcholine, Cardiac microdialysis, Rabbits, Vagal stimulation",

author = "Toru Kawada and Tsuyoshi Akiyama and Shuji Shimizu and Atsunori Kamiya and Kazunori Uemura and Yusuke Sata and Mikiyasu Shirai and Masaru Sugimachi",

note = "Funding Information: This study was supported by Health and Labour Sciences Research Grants ( H18-nano-Ippan-003 , H19-nano-Ippan-009 , H20-katsudo-Shitei-007 , and H21-nano-Ippan-005 ) from the Ministry of Health, Labour and Welfare of Japan ; by a Grant-in-Aid for Scientific Research ( No. 20390462 ) from the Ministry of Education, Culture, Sports, Science and Technology of Japan ; and by the Industrial Technology Research Grant Program from the New Energy and Industrial Technology Development Organization (NEDO) of Japan .",

year = "2010",

month = aug,

doi = "10.1016/j.autneu.2010.04.003",

language = "English",

volume = "156",

pages = "149--151",

journal = "Autonomic Neuroscience: Basic and Clinical",

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TY - JOUR

T1 - Large conductance Ca2+-activated K+ channels inhibit vagal acetylcholine release at the rabbit sinoatrial node

AU - Kawada, Toru

AU - Akiyama, Tsuyoshi

AU - Shimizu, Shuji

AU - Kamiya, Atsunori

AU - Uemura, Kazunori

AU - Sata, Yusuke

AU - Shirai, Mikiyasu

AU - Sugimachi, Masaru

N1 - Funding Information: This study was supported by Health and Labour Sciences Research Grants ( H18-nano-Ippan-003 , H19-nano-Ippan-009 , H20-katsudo-Shitei-007 , and H21-nano-Ippan-005 ) from the Ministry of Health, Labour and Welfare of Japan ; by a Grant-in-Aid for Scientific Research ( No. 20390462 ) from the Ministry of Education, Culture, Sports, Science and Technology of Japan ; and by the Industrial Technology Research Grant Program from the New Energy and Industrial Technology Development Organization (NEDO) of Japan .

PY - 2010/8

Y1 - 2010/8

N2 - Although large conductance Ca2+-activated K+ (BK) channels play an important role in determining vascular tone, their role in the efferent cardiac vagal system remains to be elucidated. In anesthetized rabbits (n=9), acetylcholine (ACh) was measured at the right atrium near the sinoatrial node by a cardiac microdialysis technique, and the ACh release in response to electrical stimulation of the cervical preganglionic vagal nerves was examined. Local administration of a BK channel blocker iberiotoxin (2μM) through a dialysis fiber increased the stimulation-induced ACh release from 7.6±2.7 to 9.0±3.2nM (P<0.05). Addition of intravenous administration of iberiotoxin (0.11mg/body) did not increase the stimulation-induced ACh release further (10.8±4.4nM). These results indicate that the BK channels play an inhibitory role in the vagal ACh release to the sinoatrial node.

AB - Although large conductance Ca2+-activated K+ (BK) channels play an important role in determining vascular tone, their role in the efferent cardiac vagal system remains to be elucidated. In anesthetized rabbits (n=9), acetylcholine (ACh) was measured at the right atrium near the sinoatrial node by a cardiac microdialysis technique, and the ACh release in response to electrical stimulation of the cervical preganglionic vagal nerves was examined. Local administration of a BK channel blocker iberiotoxin (2μM) through a dialysis fiber increased the stimulation-induced ACh release from 7.6±2.7 to 9.0±3.2nM (P<0.05). Addition of intravenous administration of iberiotoxin (0.11mg/body) did not increase the stimulation-induced ACh release further (10.8±4.4nM). These results indicate that the BK channels play an inhibitory role in the vagal ACh release to the sinoatrial node.

KW - Acetylcholine

KW - Cardiac microdialysis

KW - Rabbits

KW - Vagal stimulation

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