TY - JOUR
T1 - Large-scale preparation of Asn-glycopeptide carrying structurally homologous antigenic N-glycan
AU - Maeda, Megumi
AU - Takeda, Naoto
AU - Mano, Aya
AU - Yamanishi, Maomi
AU - Kimura, Mariko
AU - Kimura, Yoshinobu
N1 - Funding Information:
This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Basic Research C, no. 24580494 to M. M., no. 23500944 to M. K., and no. 24580495 to Y. K.) and Program to disseminate tenure tracking system (FY 2011–2013) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to M. M.). We are grateful to the Department of Instrumental Analysis, Advanced Science Research Center, Okayama University, for ESI-MS analysis.
PY - 2013
Y1 - 2013
N2 - In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770-778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asnglycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.
AB - In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770-778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asnglycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.
KW - Ginkgo biloba
KW - Glycopeptide preparation
KW - Immunoreactive glycan
KW - Neoglycoconjugate
KW - Plant N-glycan
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U2 - 10.1271/bbb.130076
DO - 10.1271/bbb.130076
M3 - Article
C2 - 23748790
AN - SCOPUS:84880179069
SN - 0916-8451
VL - 77
SP - 1269
EP - 1274
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 6
ER -