Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel

Ryota Takeuchi; Megumi Maeda; Miran Nakano; Hiroaki Funahashi; Yoshinobu Kimura

doi:10.1093/bbb/zbab114

Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel

Ryota Takeuchi, Megumi Maeda, Miran Nakano, Hiroaki Funahashi, Yoshinobu Kimura

Research output: Contribution to journal › Article › peer-review

Abstract

Sialyl-Tn antigen, a tumor antigen, is a valuable ligand for the purification of proteins that specifically bind to it. Here, we developed a new method for the preparation of large amounts of sialyl-Tn antigen-containing peptides from an unused resource, boar seminal gel. The glycopeptides were prepared from the actinase E digests by a combination of gel filtration and hydrophilic partitioning.

Original language	English
Pages (from-to)	2022-2025
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	85
Issue number	9
DOIs	https://doi.org/10.1093/bbb/zbab114
Publication status	Published - Sept 1 2021

Keywords

O-glycans
boar seminal gel
mucin-like glycoproteins
sialyl-Tn antigens

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1093/bbb/zbab114

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abstract = "Sialyl-Tn antigen, a tumor antigen, is a valuable ligand for the purification of proteins that specifically bind to it. Here, we developed a new method for the preparation of large amounts of sialyl-Tn antigen-containing peptides from an unused resource, boar seminal gel. The glycopeptides were prepared from the actinase E digests by a combination of gel filtration and hydrophilic partitioning. ",

keywords = "O-glycans, boar seminal gel, mucin-like glycoproteins, sialyl-Tn antigens",

author = "Ryota Takeuchi and Megumi Maeda and Miran Nakano and Hiroaki Funahashi and Yoshinobu Kimura",

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AU - Takeuchi, Ryota

AU - Maeda, Megumi

AU - Nakano, Miran

AU - Funahashi, Hiroaki

AU - Kimura, Yoshinobu

N1 - Funding Information: This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Basic Research C, grant number 18K05559 to M.M. and grant number 20K05959 to Y.K.). Publisher Copyright: © 2021 The Author(s). Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.

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N2 - Sialyl-Tn antigen, a tumor antigen, is a valuable ligand for the purification of proteins that specifically bind to it. Here, we developed a new method for the preparation of large amounts of sialyl-Tn antigen-containing peptides from an unused resource, boar seminal gel. The glycopeptides were prepared from the actinase E digests by a combination of gel filtration and hydrophilic partitioning.

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KW - O-glycans

KW - boar seminal gel

KW - mucin-like glycoproteins

KW - sialyl-Tn antigens

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Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel

Abstract

Keywords

ASJC Scopus subject areas

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