Levels of human defensin-1, an antimicrobial peptide, in saliva of patients with oral inflammation

Nobuyoshi Mizukawa, Katsumi Sugiyama, Takaaki Uenokatsuaki Mishima, Shin Takagi, Toshio Sugahara

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)


Objective. The purpose of this study was to investigate the presence of an antimicrobial peptide, human defensin-1, in the saliva of patients with oral inflammation. Study design. Whole saliva samples were collected from patients with oral inflammation and from healthy volunteers. Human defensin-1 in saliva was isolated and purified by reversed-phase high-performance liquid chromatography. The amino acid sequence and molecular weight of defensin-1 were determined. The concentration of defensin-1 in saliva was quantified by high-performance liquid chromatography. Serum C-reactive protein concentration was measured by particle-enhanced turbidi-metric immunoassay. Results. The salivary defensin-1 concentration was significantly higher in patients with oral inflammation than in healthy volunteers; furthermore, in patients with oral inflammation, the concentration was significantly higher before treatment than after treatment. In the patients with oral inflammation, there was a strong positive correlation between salivary defensin-1 concentration and serum C-reactive protein concentration. Conclusions. The findings suggest that defensin-1 in saliva may be a convenient marker of inflammation associated with oral disease.

Original languageEnglish
Pages (from-to)539-543
Number of pages5
JournalOral surgery, oral medicine, oral pathology, oral radiology, and endodontics
Issue number5
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)


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