Leydig cell hyperplasia in an ENU-induced mutant mouse with germ cell depletion

Maryam Khalaj, Abdol Rahim Abbasi, Ryo Nishimura, Kouyou Akiyama, Takehito Tsuji, Junko Noguchi, Kiyoshi Okuda, Tetsuo Kunieda

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Repro22 is an N-ethyl-N-nitrosourea (ENU)-induced mutation in mice showing depletion of both male and female germ cells. In the present study, we investigated the male phenotypes of the mutant mouse at the adult stage. The repro22/repro22 homozygous mice showed reduced body weights as well as markedly reduced testis weights. Histological examination of the testes at 4 and 10 months of age showed no germ cells in the seminiferous tubules of the affected testis while a number of Sertoli cells were observed in the tubules. In addition to the germ cell depletion, the testes of the affected mouse contained expanded intertubular spaces that were filled by Leydig cell-like interstitial cells. These interstitial cells were confirmed to be Leydig cells by immunohistochmical staining using anti-3β-HSD antibody. The estimated number of Leydig cells in the affected testes at 10 months of age increased approximately 2 fold compared with those of normal testes. Furthermore, the plasma testosterone levels of the affected mice at 10 months of age were significantly higher than those of the normal mice. These findings indicated that the repro22/repro22 mouse developed hyperplasia of Leydig cells that was presumably caused by the absence of germ cells in the seminiferous tubules.

Original languageEnglish
Pages (from-to)225-228
Number of pages4
JournalJournal of Reproduction and Development
Issue number3
Publication statusPublished - Jun 2008


  • ENU mutagenesis
  • Germ cell
  • Infertility
  • Leydig cell
  • Mouse
  • Testosterone

ASJC Scopus subject areas

  • Animal Science and Zoology


Dive into the research topics of 'Leydig cell hyperplasia in an ENU-induced mutant mouse with germ cell depletion'. Together they form a unique fingerprint.

Cite this