@article{bdba215065cb4b7b813b5b4509a3ed37,
title = "Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting",
abstract = "A pair of ankyrin repeat proteins in chloroplasts recognizes signal peptides on thylakoid-bound cargo proteins and undergoes liquid-liquid phase separation to facilitate their sorting and transport.",
keywords = "ankyrin-repeat proteins, cargo sorting, chloroplast, intrinsically disordered regions, liquid-liquid phase separation, nuclear-encoded chloroplast proteins, thylakoid membranes, twin arginine translocation pathway",
author = "Min Ouyang and Xiaoyi Li and Jing Zhang and Peiqiang Feng and Hua Pu and Lingxi Kong and Zechen Bai and Liwei Rong and Xiumei Xu and Wei Chi and Qiang Wang and Fan Chen and Congming Lu and Jianren Shen and Lixin Zhang",
note = "Funding Information: We thank Professor Jean-David Rochaix and Professor Lei Wang for assisting in writing and discussion. We thank Professor Tom Rapoport for giving us valuable suggestions. We thank the Shanghai Synchrotron Radiation Facility (SSRF) BL17U. We thank Engineer Jingquan Li for technical assistance. This study was supported by the National Natural Science Foundation of China (31471267 and 31130059), the State Key Basic Research and Development Plan of China (2015CB150100), the National Key Research and Development Program of China (2017YFA0503700), the Outstanding Talents Fund of Henan University, China, the State Key Laboratory of Biocatalysis and Enzyme Engineering of Hubei University, China, and the Youth Innovation Promotion Association CAS, China (2013059). M.O. and L.Z. designed the study. M.O. X.L. J.Z. P.F. H.P. L.K. Z.B. L.R. X.X. W.C. Q.W. F.C. C.L, J.S. and L.Z. performed the research and analyzed the data. M.O. and L.Z. wrote the paper, and all authors commented on it. F.C. Q.W. and C.L assisted in the writing and discussion. The authors declare no competing interests. Funding Information: We thank Professor Jean-David Rochaix and Professor Lei Wang for assisting in writing and discussion. We thank Professor Tom Rapoport for giving us valuable suggestions. We thank the Shanghai Synchrotron Radiation Facility (SSRF) BL17U. We thank Engineer Jingquan Li for technical assistance. This study was supported by the National Natural Science Foundation of China ( 31471267 and 31130059 ), the State Key Basic Research and Development Plan of China ( 2015CB150100 ), the National Key Research and Development Program of China ( 2017YFA0503700 ), the Outstanding Talents Fund of Henan University , China, the State Key Laboratory of Biocatalysis and Enzyme Engineering of Hubei University , China, and the Youth Innovation Promotion Association CAS , China ( 2013059 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = mar,
day = "19",
doi = "10.1016/j.cell.2020.02.045",
language = "English",
volume = "180",
pages = "1144--1159.e20",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}