Localization of PTP-FERM in nerve processes through its FERM domain

Yohzo Uchida; Masato Ogata; Yoshiko Mori; Masatsugu Oh-hora; Naoya Hatano; Toshiyuki Hamaoka

doi:10.1006/bbrc.2002.6613

Localization of PTP-FERM in nerve processes through its FERM domain

Yohzo Uchida, Masato Ogata, Yoshiko Mori, Masatsugu Oh-hora, Naoya Hatano, Toshiyuki Hamaoka

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.

Original language	English
Pages (from-to)	13-19
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	292
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.2002.6613
Publication status	Published - Jan 1 2002
Externally published	Yes

Keywords

C. elegans
FERM domain
Nerve processes
Protein-tyrosine-phosphatase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.",

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AU - Uchida, Yohzo

AU - Ogata, Masato

AU - Mori, Yoshiko

AU - Oh-hora, Masatsugu

AU - Hatano, Naoya

AU - Hamaoka, Toshiyuki

PY - 2002/1/1

Y1 - 2002/1/1

N2 - PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.

AB - PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.

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Localization of PTP-FERM in nerve processes through its FERM domain

Abstract

Keywords

ASJC Scopus subject areas

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