Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

Masayoshi Mishina; Takamasa Tobimatsu; Keiji Imoto; Ken Ichi Tanaka; Yoshihiko Fujita; Kazuhiko Fukuda; Masaaki Kurasaki; Hideo Takahashi; Yuuki Morimoto; Tadaaki Hirose; Seiichi Inayama; Tomoyuki Takahashi; Motoy Kuno; Shosaku Numa

doi:10.1038/313364a0

Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

Masayoshi Mishina, Takamasa Tobimatsu, Keiji Imoto, Ken Ichi Tanaka, Yoshihiko Fujita, Kazuhiko Fukuda, Masaaki Kurasaki, Hideo Takahashi, Yuuki Morimoto, Tadaaki Hirose, Seiichi Inayama, Tomoyuki Takahashi, Motoy Kuno, Shosaku Numa

Research output: Contribution to journal › Article › peer-review

275 Citations (Scopus)

Abstract

The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

Original language	English
Pages (from-to)	364-369
Number of pages	6
Journal	Nature
Volume	313
Issue number	6001
DOIs	https://doi.org/10.1038/313364a0
Publication status	Published - Dec 1 1985
Externally published	Yes

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title = "Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis",

abstract = "The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.",

author = "Masayoshi Mishina and Takamasa Tobimatsu and Keiji Imoto and Tanaka, {Ken Ichi} and Yoshihiko Fujita and Kazuhiko Fukuda and Masaaki Kurasaki and Hideo Takahashi and Yuuki Morimoto and Tadaaki Hirose and Seiichi Inayama and Tomoyuki Takahashi and Motoy Kuno and Shosaku Numa",

year = "1985",

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doi = "10.1038/313364a0",

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T1 - Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

AU - Mishina, Masayoshi

AU - Tobimatsu, Takamasa

AU - Imoto, Keiji

AU - Tanaka, Ken Ichi

AU - Fujita, Yoshihiko

AU - Fukuda, Kazuhiko

AU - Kurasaki, Masaaki

AU - Takahashi, Hideo

AU - Morimoto, Yuuki

AU - Hirose, Tadaaki

AU - Inayama, Seiichi

AU - Takahashi, Tomoyuki

AU - Kuno, Motoy

AU - Numa, Shosaku

PY - 1985/12/1

Y1 - 1985/12/1

N2 - The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

AB - The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

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ER -

Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

Abstract

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