Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5–30 kV and a specimen current of 1 x 10-10A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutar-aldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).
|Number of pages||9|
|Journal||Archivum Histologicum Japonicum|
|Publication status||Published - 1987|
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