Lysine-Mediated Tissue Osmication in Combination with a Tannin-Osmium Conductive Staining Method for Non-Coated Scanning Electron Microscopy of Biological Specimens

Takuro Murakami; Zhen lan Song; Hitoshi Hinenoya; Aiji Ohtsuka; Takehito Taguchi; Jing jie Liu; Tadashi Sano

doi:10.1679/aohc.50.485

Lysine-Mediated Tissue Osmication in Combination with a Tannin-Osmium Conductive Staining Method for Non-Coated Scanning Electron Microscopy of Biological Specimens

Takuro Murakami, Zhen lan Song, Hitoshi Hinenoya, Aiji Ohtsuka, Takehito Taguchi, Jing jie Liu, Tadashi Sano

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5–30 kV and a specimen current of 1 x 10^-10A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutar-aldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).

Original language	English
Pages (from-to)	485-493
Number of pages	9
Journal	Archivum Histologicum Japonicum
Volume	50
Issue number	5
DOIs	https://doi.org/10.1679/aohc.50.485
Publication status	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Anatomy
Histology

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title = "Lysine-Mediated Tissue Osmication in Combination with a Tannin-Osmium Conductive Staining Method for Non-Coated Scanning Electron Microscopy of Biological Specimens",

abstract = "Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5–30 kV and a specimen current of 1 x 10-10A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutar-aldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).",

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language = "English",

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AU - Murakami, Takuro

AU - Song, Zhen lan

AU - Hinenoya, Hitoshi

AU - Ohtsuka, Aiji

AU - Taguchi, Takehito

AU - Liu, Jing jie

AU - Sano, Tadashi

PY - 1987

Y1 - 1987

N2 - Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5–30 kV and a specimen current of 1 x 10-10A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutar-aldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).

AB - Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5–30 kV and a specimen current of 1 x 10-10A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutar-aldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).

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Lysine-Mediated Tissue Osmication in Combination with a Tannin-Osmium Conductive Staining Method for Non-Coated Scanning Electron Microscopy of Biological Specimens

Abstract

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